Related Experiment Video For circulating tumor cells (CTCs)
Updated: Jan 24, 2026

Induction and Analysis of Epithelial to Mesenchymal Transition
Published on: August 27, 2013
Dual-antibody Modified PLGA Nanofibers for Specific Capture of Epithelial and Mesenchymal CTCs
Hui Liu1, Zhili Wang2, Changchong Chen1
1CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China; Nano Science and Technology Institute, University of Science and Technology of China, Suzhou, 215123, China.
Abstract:
CTC detection has great potential to provide crucial clinical information for early cancer diagnosis, patient prognosis, personalized therapy, and cancer progress monitoring etc. It has been proved that the disease progress is associated with an increase in mesenchymal CTCs, while most mature techniques have been developed based on epithelial CTC detection. In present work, a dual-antibody nanointerface against EpCAM and N-Cadherin was developed to capture epithelial CTCs as well as mesenchymal CTCs from blood samples. A uniform poly-(lactic-co-glycolic acid) (PLGA) nanofiber substrate was fabricated by electrospinning to provide a platform for cell capture (i.e., MCF-7 and GIST882 cells), modified with BSA and dual antibodies. Our results showed the dual-antibody substrates exhibited an improved capture efficiency of target cells compared to the mono-antibody ones, revealing potential application of the dual-antibody PLGA nanofibers for efficient capture of epithelial and mesenchymal CTCs in clinic.
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