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Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway
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CD28 deficiency attenuates primary blast-induced renal injury in mice via the PI3K/Akt signalling pathway

Ying Liu1, Y E Liu1, C C Tong1

  • 1Emergency Medicine Department of General Hospital of Northern Theater Command, Laboratory of Rescue Center of Severe Wound and Trauma PLA, Shenyang, China.

BMJ Military Health
|May 27, 2019
PubMed
Abstract

Insights

CD28 deficiency protects kidneys from primary blast injury by reducing inflammation and altering the PI3K/Akt pathway. This finding offers a potential therapeutic target for blast-induced renal damage.

Area of Science:

  • Trauma research
  • Immunology
  • Renal pathophysiology

Background:

  • Primary blast injury causes kidney damage via shock waves and inflammation.
  • CD28 plays a role in T cell activation and inflammatory cytokine secretion.
  • Effective treatments for blast-induced kidney injury are lacking.

Purpose of the Study:

  • Investigate the impact of primary blast on kidney injury in mice.
  • Determine the role of CD28 in modulating blast-induced renal damage.

Main Methods:

  • Established a mouse model of primary blast-induced kidney injury.
  • Assessed renal injury severity using H&E staining.
  • Quantified inflammatory markers (IL-1β, IL-4, IL-6, IL-10) via ELISA and Western blot.
  • Examined the PI3K/Akt signaling pathway using immunofluorescence.

Main Results:

  • CD28 deficiency significantly reduced the severity of primary blast-induced kidney injury.
  • CD28 deficiency decreased pro-inflammatory cytokines (IL-1β, IL-4, IL-6) and increased anti-inflammatory IL-10.
  • Changes in PI3K/Akt signaling pathway expression were observed in CD28-deficient mice.

Conclusions:

  • CD28 deficiency confers protection against primary blast-induced kidney injury.
  • The protective mechanism involves modulation of the PI3K/Akt signaling pathway.
  • Findings provide a theoretical basis for developing treatments for blast-induced renal injury.
Keywords:
CD28kidney injurypi3k/aktprimary blast

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