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The Difference in Cytotoxic Activity between Two Optical Isomers of Gelsemine from Gelsemium elegans Benth. on PC12
Li Lin1, Yan-Chun Liu2,3, Zhao-Ying Liu4,5
1College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China. linli.1110@foxmail.com.
Abstract:
Two optical isomers, +/- gelsemine (1, 2), together with one known compound were isolated from the whole plant of G. elegans. The structures of the separated constituents were elucidated on 1D and 2D (1H-1H COSY, HMBC, HSQC) NMR spectroscopy and high-resolution mass spectrometry (HRMS). The isolated alkaloids were tested in vitro for cytotoxic potential against PC12 cells by the MTT assay. As a result, (+) gelsemine (compound 1) exhibited cytotoxic activity against PC12 cells with an IC50 value of 31.59 μM, while (-) gelsemine (compound 2) was not cytotoxic.
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