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A high-throughput amplicon-based method for estimating outcrossing rates.

Friederike Jantzen1, Natalia Wozniak1,2, Christian Kappel1

  • 11Institute for Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, House 26, 14476 Potsdam-Golm, Germany.

Plant Methods
|May 28, 2019
PubMed
Summary
This summary is machine-generated.

We developed a new, high-throughput method to estimate the outcrossing rate in plants. This technique efficiently measures genetic outcrossing, aiding evolutionary and genetic studies.

Keywords:
Amplicon sequencingCapsellaMixed matingOutcrossingOutcrossing rate

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Area of Science:

  • Population genetics
  • Evolutionary biology
  • Plant reproductive biology

Background:

  • The outcrossing rate significantly influences a species' population-genetic structure and evolutionary path.
  • Current methods for determining outcrossing rates are labor-intensive and time-consuming, relying on PCR-genotyping of numerous offspring and markers.

Purpose of the Study:

  • To develop and validate a novel, high-throughput method for estimating plant outcrossing rates.
  • To address the limitations of existing laborious techniques for measuring outcrossing.

Main Methods:

  • An amplicon-based, high-throughput enabled method was developed.
  • The method was applied to scented versus non-scented *Capsella* genotypes to estimate outcrossing rates.

Main Results:

  • The new method robustly captures differences in outcrossing rates between genotypes.
  • Potential biases in estimates due to differential haplotype sharing were identified.

Conclusions:

  • This high-throughput method facilitates the estimation of outcrossing rates across numerous genotypes.
  • Enables large-scale studies on the genetic factors influencing pollinator attraction and outcrossing success.