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Updated: Jan 24, 2026

Oxygen-Induced Retinopathy Model for Ischemic Retinal Diseases in Rodents
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Oxygen-Induced Retinopathy Model for Ischemic Retinal Diseases in Rodents

Published on: September 16, 2020

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Identification of altered microRNAs in retinas of mice with oxygen-induced retinopathy

Lu-Si Zhang1,2, Ye-Di Zhou1,2, Ying-Qian Peng1,2

  • 1Department of Ophthalmology, the Second Xiangya Hospital of Central South University, Changsha 410011, Hunan Province, China.

International Journal of Ophthalmology
|May 28, 2019
PubMed
Abstract

Insights

Researchers identified specific microRNAs (miRNAs) altered in mouse retinas with oxygen-induced retinopathy (OIR). These differentially expressed miRNAs may offer novel therapeutic targets for treating pathological retinal neovascularization.

Area of Science:

  • Ophthalmology
  • Molecular Biology
  • Genetics

Background:

  • Oxygen-induced retinopathy (OIR) is a significant cause of pathological neovascularization in the retina.
  • MicroRNAs (miRNAs) play crucial roles in regulating gene expression and are implicated in various disease processes.
  • Understanding miRNA dysregulation in OIR is essential for developing targeted therapies.

Purpose of the Study:

  • To identify disease-related miRNAs in the retinas of mice with OIR.
  • To investigate the potential roles of these miRNAs in retinal pathological neovascularization.

Main Methods:

  • miRNA expression profiling using microarray analysis in OIR mouse models and controls.
  • Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) for validating miRNA expression.
  • Bioinformatic analyses including Targetscan7.1, MirdbV5, Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.

Main Results:

  • 3 miRNAs were significantly upregulated and 8 were significantly downregulated in OIR retinas compared to controls.
  • qRT-PCR confirmed upregulation of mmu-miR-350-3p and mmu-miR-202-3p, and downregulation of mmu-miR-711 and mmu-miR-30c-1-3p.
  • GO and KEGG analyses indicated involvement of target genes in cellular metabolism, Wnt signaling, cancer pathways, and MAPK signaling.

Conclusions:

  • Differentially expressed miRNAs in OIR retinas are identified.
  • These miRNAs represent potential therapeutic targets for retinal neovascularization.
Keywords:
microRNAsmicroarrayoxygen-induced retinopathyretinal neovascularization

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