Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

What are Viruses?00:50

What are Viruses?

127.9K
Overview
127.9K
Self-Evaluation: Self-Enhancement and Self-Verification03:00

Self-Evaluation: Self-Enhancement and Self-Verification

5.8K
Social psychologists have documented that feeling good about ourselves and maintaining positive self-esteem is a powerful motivator of human behavior (Tavris & Aronson, 2008). In the United States, members of the predominant culture typically think very highly of themselves and view themselves as good people who are above average on many desirable traits (Ehrlinger, Gilovich, & Ross, 2005). Often, our behavior, attitudes, and beliefs are affected when we experience a threat to our...
5.8K
Bioavailability Enhancement: Drug Solubility Enhancement01:16

Bioavailability Enhancement: Drug Solubility Enhancement

236
Body:Bioavailability is a critical factor in determining a drug's effectiveness. It refers to the proportion of a drug that enters the circulation when introduced into the body and is, as a result, able to have an active effect. Enhancing bioavailability is essential for drugs with poor solubility, as it can significantly impact their therapeutic efficacy. Various methods are employed to increase the solubility of drugs, thereby enhancing their bioavailability.Micronization and nanonization are...
236
Bioavailability Enhancement: Drug Permeability Enhancement01:27

Bioavailability Enhancement: Drug Permeability Enhancement

195
Body:After oral administration, poor permeability often limits the rate at which drugs are absorbed through the intestinal epithelium. Enhancing drug permeability is crucial for effective therapy, and several strategies have been developed to overcome this challenge.One effective strategy involves the use of lipid-based formulations. These formulations enhance dissolution and solubility, targeting physiological mechanisms to increase drug absorption. This includes stimulating bile salt...
195
Bioavailability Enhancement: Drug Stability Enhancement and GI Retention01:05

Bioavailability Enhancement: Drug Stability Enhancement and GI Retention

204
Body:Improving a drug's stability in the gastrointestinal (GI) tract is paramount for enhancing its bioavailability and therapeutic effectiveness. Various strategies are employed to protect the drug from the harsh gastric milieu and to ensure its release and absorption at the desired site within the GI tract.Polymer coatings are one such method used to shield drugs from the stomach's acidic environment. By preventing premature drug release, these coatings improve the bioavailability of unstable...
204
MOSFET: Enhancement Mode01:22

MOSFET: Enhancement Mode

807
Enhancement-mode MOSFETs are pivotal components in electronics, distinguished by their capacity to act as highly efficient switches. They are part of the larger family of metal-oxide Semiconductor Field-Effect Transistors (MOSFETs). They are available in two types: p-channel and n-channel, each tailored to specific polarity operations.
In their basic form, enhancement-mode MOSFETs are typically non-conductive when the gate-source voltage (Vgs) is zero. This default 'off' state means no...
807

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nanofiber Length Shapes Vaccine Immunity: Mechanistic Insights from M13 Bacteriophage.

Nano letters·2026
Same author

Patchouli oil and its main component patchouli alcohol inhibit colorectal cancer by activating ectopic olfactory receptor.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026
Same author

Immunotherapy response and resistance in endometrial cancer: Integrating molecular subtype, spatial immune states, and pharmacodynamic biomarkers.

Critical reviews in oncology/hematology·2026
Same author

Histone deacetylase BrHDA19 is involved in regulating bolting time in Chinese cabbage.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2026
Same author

Identification of a new male sterile gene BrATAF1 of Chinese cabbage.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2026
Same author

Denitrification kinetics and microbial evolution of anaerobic ammonia oxidation for low-acidity ammonia nitrogen wastewater leached from rare earth tailings.

Environmental technology·2026
Same journal

A Droplet-Microarray Platform for Multiplex Profiling of Breast Cancer Exosome Subtypes in Patients' Blood Plasma Samples.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Material-Dependent Functionalization of CVD-Grown TMDC Monolayers Probed by Vibrational Nanospectroscopy.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

BandGap Modulated Charge Gating of Semiconductor Coatings Stabilizes Zinc Metal Anodes.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

For High Capacity: Upcycling of Spent Graphite Catalytic via Precisely Tailoring Water-Gas Reaction.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Electronic Engineering of Donor-Acceptor Covalent Organic Frameworks via Fluorine Substitution for Efficient Solar Hydrogen Production.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Correction to: "A Gold Nanocage/Cluster Hybrid Structure for Whole-Body Multispectral Optoacoustic Tomography Imaging, EGFR Inhibitor Delivery, and Photothermal Therapy".

Small (Weinheim an der Bergstrasse, Germany)·2026
See all related articles

Related Experiment Video

Updated: Jan 24, 2026

A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum
07:06

A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum

Published on: April 10, 2018

9.1K

M13 Virus-Based Framework for High Fluorescence Enhancement.

Shengnan Huang1,2, Jifa Qi2,3, Dane W deQuilettes4

  • 1Department of Materials Science and Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA, 02139, USA.

Small (Weinheim an Der Bergstrasse, Germany)
|May 28, 2019
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel fluorescent tag using M13 virus and silver nanoparticles to significantly enhance fluorescence imaging. This new probe offers high precision and improved detection limits for biological molecules.

Keywords:
M13 virusfluorescence enhancementfluorescence imagingsurface plasmons

More Related Videos

Constructing and Visualizing Models using Mime-based Machine-learning Framework
06:19

Constructing and Visualizing Models using Mime-based Machine-learning Framework

Published on: July 22, 2025

2.4K
Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals
09:31

Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals

Published on: April 15, 2017

19.0K

Related Experiment Videos

Last Updated: Jan 24, 2026

A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum
07:06

A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum

Published on: April 10, 2018

9.1K
Constructing and Visualizing Models using Mime-based Machine-learning Framework
06:19

Constructing and Visualizing Models using Mime-based Machine-learning Framework

Published on: July 22, 2025

2.4K
Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals
09:31

Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals

Published on: April 15, 2017

19.0K

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Molecular Imaging

Background:

  • Fluorescence imaging is crucial for studying biomolecules but limited by probe specificity and efficiency.
  • M13 virus scaffolds offer precise assembly and cell targeting but suffer from low detection limits.
  • Existing M13-fluorophore systems have limitations due to low absorption and moderate quantum yields.

Purpose of the Study:

  • To develop a highly emissive fluorescent tag with molecular precision.
  • To overcome the limitations of low detection limits in M13-fluorophore systems.
  • To create an inexpensive framework for enhanced fluorescence detection.

Main Methods:

  • Coassembly of M13 virus, cyanine 3 dye, and silver nanoparticles.
  • Tuning nanoparticle size and particle-fluorophore separation for emission enhancement.
  • Investigating the effect of dye surface density on fluorescence.

Main Results:

  • Achieved up to 24-fold enhanced emission of cyanine 3.
  • Demonstrated that fluorescence enhancement depends on nanoparticle size and dye density.
  • Successfully applied the probe for in vitro staining of E. coli.

Conclusions:

  • Developed a novel, inexpensive fluorescent tag with enhanced emission and molecular precision.
  • The methodology provides a tunable framework for improved fluorescence detection.
  • This approach is adaptable for various M13/cell combinations in biological imaging.