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Updated: Jan 24, 2026

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP
Published on: September 8, 2023
Development and evaluation of a one-step reverse transcription loop-mediated isothermal amplification for detection
Huan Liu1, Wei Wu2, Jiaqi Tan1
1State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Key Laboratory of Crop Pest Integrated Pest Management on Crop in Northwestern Loess Plateau, Ministry of Agriculture, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Abstract:
Citrus leaf blotch virus (CLBV) is a type member of the genus Citrivirus belonging to Betaflexiviridae. In this study, a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method was developed to detect CLBV; this technology has been widely used in the detection of various plant pathogenic microorganisms and exogenous genes. The sensitivity of the RT-LAMP method was increased 100-fold compared to that of the conventional RT-PCR. In addition, this method was fast, simple and specific; it could provide better technical support for field diagnosis, customs quarantine and the control measures of CLBV. To our knowledge, this is the first report detecting CLBV using RT-LAMP.
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