[Determination of amino acids in Hulless barley]

A new method for the determination of 16 amino acids in Hulless barley which were planted in the Tibetan plateau of China was established by the amino acid analyzer(AAA), and the amino acid was graded.

The samples were subjected to hydrolysis by the hydrochloric acid solution containing 0. 05% thioglycolic acid, and were carried out by AAA and hydrolyzed amino acid column PH(4. 6 mm×60 mm, 3 μm). The external standard method was used for quantitative analysis.

This hydrolysis pretreatment process could effectively prevent oxidation of methionine. The recoveries were 93. 2%-96. 7%, and the relative standard deviations were no more than 2. 9%(n=6). As the cereal-restricted amino acid, Hulless barley restricted amino acid lysine was the content of up to 0. 367 g/100 g, and the lysine score(AAS) was 62. 4, which was better than the corresponding scores of wheat, glutinous rice, corn and millet.

The method is accurate, and has good repeatability which could meet the requirements for determination of 16 amino acids in Hulless barley.