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Canine amniotic membrane mesenchymal stromal/stem cells: Isolation, characterization and differentiation
Jéssica Borghesi1, Mariana Ferreira Lima2, Lara Carolina Mario1
1Department of Surgery, School of Veterinary Medicine and Animal Science, University of Sao Paulo (FMVZ-USP), Sao Paulo, Brazil.
Tissue & Cell
|May 29, 2019
Summary
Canine amniotic membrane (AMC) is a novel source for isolating mesenchymal stromal/stem cells (MSCs). These canine MSCs exhibit low immunogenicity and tumorigenic potential, making them promising for veterinary regenerative medicine.
Area of Science:
- Veterinary Medicine
- Stem Cell Biology
- Immunology
Background:
- The amniotic membrane, located at the fetal-maternal interface, is recognized for its low immunogenicity.
- Mesenchymal stromal/stem cells (MSCs) have not been previously identified in canine amniotic membrane (AMC).
Purpose of the Study:
- To isolate, culture, characterize, and differentiate cells from canine amniotic membrane (AMC).
- To evaluate the immunological and tumorigenic potential of these derived cells for veterinary therapeutic applications.
Main Methods:
- Isolation and culture of cells from canine amniotic membrane (AMC) across different gestational ages (32, 43, 55 days).
- Characterization of cell morphology, differentiation potential (osteogenic, adipogenic, chondrogenic), immunophenotype (CD34, CD105), and immunological markers (ILs, MHC).
- Tumorigenicity assessment through application of AMC cells in nude mice.
Main Results:
- Cells isolated from canine AMC displayed fibroblastoid morphology and high confluence post-cryopreservation.
- Induced differentiation confirmed osteogenic, adipogenic, and chondrogenic capabilities.
- Immunophenotyping revealed CD34- and CD105+ expression. Immunological analysis showed no expression of IL-1, IL-2, IL-6, IL-10, and MHC II, but expressed MHC I.
- No tumor formation was observed in nude mice post-application of AMC cells.
Conclusions:
- Canine amniotic membrane (AMC) serves as an accessible source for mesenchymal stromal/stem cells (MSCs).
- These canine MSCs possess low immunogenic and tumorigenic properties.
- AMC-derived MSCs hold significant potential for veterinary therapeutic applications.
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