Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Guinea-pig lymphotoxin resistant L-cell sublines.

Y Kobayashi, J Sawada, T Osawa

    Immunology
    |January 1, 1979
    PubMed
    Summary

    Researchers identified guinea-pig lymphotoxin (GLT)-resistant cells lacking a specific 140,000 MW glycoprotein. This suggests the glycoprotein is the GLT receptor, and these cells are GLT receptor-deficient.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Local extensive granulomatous inflammation of the neck region and lymphangitis caused by <i>Lichtheimia corymbifera</i> infection in a Japanese Black calf.

    Medical mycology case reports·2018
    Same author

    Control of mammary tumor differentiation by SKI-606 (bosutinib).

    Oncogene·2010
    Same author

    Homozygous CDA*3 is a major cause of life-threatening toxicities in gemcitabine-treated Japanese cancer patients.

    British journal of cancer·2009
    Same author

    Functional characterization of CYP3A4.16: catalytic activities toward midazolam and carbamazepine.

    Xenobiotica; the fate of foreign compounds in biological systems·2009
    Same author

    Functional characterization of two novel CYP2C19 variants (CYP2C19*18 and CYP2C19*19) found in a Japanese population.

    Xenobiotica; the fate of foreign compounds in biological systems·2007
    Same author

    Ingestion of heat-treated Lactobacillus rhamnosus GG prevents development of atopic dermatitis in NC/Nga mice.

    Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2007

    Area of Science:

    • Cell biology
    • Immunology
    • Biochemistry

    Background:

    • Lymphotoxin (LT) is a cytokine involved in inflammation and immunity.
    • Understanding LT-receptor interactions is crucial for developing targeted therapies.
    • L-cell sublines resistant to GLT were generated to study LT binding.

    Purpose of the Study:

    • To identify the molecular basis of guinea-pig lymphotoxin (GLT) resistance in L-cell sublines.
    • To characterize the cell-surface alterations associated with GLT resistance.
    • To determine the potential role of specific glycoproteins in GLT binding.

    Main Methods:

    • Isolation and characterization of GLT-resistant L-cell sublines (No. 1 and No. 2).
    • Assessment of GLT absorption capacity and binding of labeled Ricinus communis agglutinin (RcA).
    • Analysis of cell-surface proteins and glycoproteins using biochemical techniques, including RcA-Sepharose affinity chromatography.

    Main Results:

    • GLT-resistant cell sublines (No. 1 and No. 2) exhibited reduced GLT absorption and RcA binding.
    • No. 2 cells showed significant differences in cell-surface proteins and glycoproteins compared to parental L cells.
    • A specific 140,000 molecular weight glycoprotein, which binds RcA, was absent in No. 2 cell extracts.

    Conclusions:

    • The 140,000 MW glycoprotein is strongly implicated as the GLT receptor molecule.
    • No. 2 cells, and likely No. 1 cells, are deficient in GLT receptors.
    • This finding provides a molecular target for understanding and potentially modulating GLT signaling.

    Related Experiment Videos