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PsRPs26, a 40S Ribosomal Protein Subunit, Regulates the Growth and Pathogenicity of Puccinia striiformis f. sp.

Bing Wang1,2, Na Song1, Chunlei Tang1

  • 1State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, China.

Frontiers in Microbiology
|May 29, 2019
PubMed

Insights

The ribosomal protein gene PsRPs26 is crucial for the growth and development of Puccinia striiformis (Pst), a pathogen affecting wheat. Silencing PsRPs26 inhibited Pst growth and urediospore production, suggesting its role in pathogenicity.

Area of Science:

  • Molecular Biology
  • Plant Pathology
  • Mycology

Background:

  • Ribosomal proteins (RPs) are vital for eukaryotic cell functions, including growth and differentiation.
  • The specific role of RPs26 in pathogenic fungi, particularly cereal rust fungi like Puccinia striiformis (Pst), remains largely uncharacterized.
  • This study focuses on the ribosomal protein gene PsRPs26 in Pst, a significant wheat pathogen.

Purpose of the Study:

  • To isolate and characterize the PsRPs26 gene from Pst.
  • To investigate the function and localization of PsRPs26 in Pst.
  • To determine the role of PsRPs26 in Pst pathogenicity and its potential as a target for disease control.

Main Methods:

  • Isolation and sequencing of the PsRPs26 gene from Pst.
  • Bioinformatic analysis to identify conserved motifs and compare with orthologs.
  • Subcellular localization studies of the PsRPs26 protein.
  • Gene expression analysis under host-inoculation conditions.
  • Host-induced gene silencing (HIGS) to knockdown PsRPs26 expression in Pst.

Main Results:

  • The PsRPs26 gene was successfully isolated from Pst and found to contain a conserved eukaryotic Y62-K70 motif.
  • PsRPs26 protein was localized to both the nucleus and cytoplasm.
  • PsRPs26 expression levels increased upon wheat seedling inoculation with Pst.
  • Knockdown of PsRPs26 via HIGS significantly inhibited Pst growth and urediospore production.

Conclusions:

  • PsRPs26 plays a critical role in the growth and development of Pst.
  • PsRPs26 is essential for the pathogenicity of Pst on wheat.
  • Targeting PsRPs26 offers a potential new strategy for controlling cereal rust diseases.

Keywords:
Puccinia striiformisRPs26growthribosomal subunitwheat

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