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The 70-Kd neisserial common antigen is a surface-exposed, antigenically stable peptidic structure
Annales De L'Institut Pasteur. Microbiology
|May 1, 1987
Summary
A stable 70-kilodalton (Kd) antigen found in Neisseria gonorrhoeae is heat-stable and located on the bacterial surface. This antigen remains consistent even after transmission between human partners, indicating its reliability for diagnostic purposes.
Area of Science:
- Microbiology
- Immunology
- Bacteriology
Background:
- Neisseria gonorrhoeae is a significant human pathogen.
- A 70-kilodalton (Kd) antigen has been identified across Neisseria species.
- The antigen's stability and location were previously uncharacterized.
Purpose of the Study:
- To characterize the 70-Kd antigen from Neisseria gonorrhoeae.
- To determine its stability and surface localization.
- To assess its antigenic stability during human transmission.
Main Methods:
- Protein characterization using mercaptoethanol and heat treatment.
- Immunological assays with mouse polyclonal antisera.
- Analysis of gonococcal strains from various anatomical sites in human partners.
Main Results:
- The 70-Kd antigen is a mercaptoethanol- and heat-stable protein.
- Antisera confirmed the antigen is exposed on the bacterial surface.
- The antigen was consistently detected in all tested Neisseria gonorrhoeae strains, including those from different anatomical sites and partners.
Conclusions:
- The 70-Kd antigen is a stable, surface-exposed protein in Neisseria gonorrhoeae.
- Its presence across diverse strains and after human transmission highlights its potential as a stable diagnostic marker.