Colorimetric and fluorescent sensing of rhodamine using polymethacrylate matrix

Area of Science:

• Analytical Chemistry
• Environmental Science

Background:

• Rhodamine dyes are widely used as tracers in hydrological studies.
• Accurate quantification of rhodamine in environmental matrices is crucial for effective tracer analysis.

Purpose of the Study:

• To develop and validate a novel method for rhodamine determination.
• To utilize solid phase extraction combined with spectrophotometric and fluorometric measurements.

Main Methods:

• Solid phase extraction of rhodamine onto a polymethacrylate matrix.
• Spectrophotometric measurements at 535 nm and fluorometric measurements at 554 nm.
• Linear calibration established for concentration ranges of 0.02-0.10 mg/L (colorimetric) and 0.002-0.06 mg/L (fluorescent).

Main Results:

• Achieved detection limits of 0.014 mg/L (colorimetric) and 0.0005 mg/L (fluorescent).
• Demonstrated linearity of calibration graphs within specified concentration ranges.
• Successfully applied the method for rhodamine determination in groundwater samples.

Conclusions:

• The developed method provides a sensitive and reliable approach for rhodamine quantification.
• This technique is suitable for environmental monitoring applications, particularly in groundwater studies.
• The combination of solid phase extraction and optical measurements offers advantages in tracer analysis.