Related Experiment Video

Updated: Jan 24, 2026

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor
07:16

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor

Published on: September 13, 2018

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[Downregulation of Herg1 suppresses osteosarcoma proliferation and invasion by targeting Hippo signaling pathway]

Z D Chen1, W B Ye1, W R Zeng1

  • 1Orthopaedics Center of PLA, the 909th Hospital of PLA, the Affiliated Southeast Hospital of Xiamen University, Zhangzhou 363000, China.

Zhonghua Zhong Liu Za Zhi [Chinese Journal of Oncology]
|May 29, 2019
PubMed

Insights

Knocking down human ether à go-go related gene 1 (Herg 1) significantly inhibited osteosarcoma cell proliferation and invasion. Herg 1 knockdown also suppressed tumor growth and metastasis in vivo, suggesting it as a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Osteosarcoma (OS) is a primary bone malignancy with limited treatment options.
  • The role of human ether à go-go related gene 1 (Herg 1) in OS progression is not fully understood.

Purpose of the Study:

  • To investigate the effect of Herg 1 knockdown on osteosarcoma cell proliferation and invasion.
  • To elucidate the regulatory mechanism of Herg 1 in osteosarcoma.

Main Methods:

  • Constructed recombinant adenovirus vector (Ad5-Herg1-shRNA) for Herg 1 knockdown.
  • Assessed proliferation, migration, and invasion using CCK-8, wound healing, and Transwell assays.
  • Utilized xenograft models, tandem affinity purification, mass spectrometry, and Western blot analysis.

Main Results:

  • Herg 1 knockdown significantly inhibited OS cell proliferation, migration, and invasion in vitro.
  • Herg 1 knockdown suppressed tumor growth and metastasis in a nude mouse xenograft model.
  • Herg 1 interacted with NF2 and regulated the LATS1/YAP signaling pathway.

Conclusions:

  • Herg 1 plays a crucial role in osteosarcoma cell proliferation and motility.
  • Herg 1 represents a potential therapeutic target for osteosarcoma.
Keywords:
Herg1Hippo signaling pathwayMotilityOsteosarcomaProliferation

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