Typing of Epidemiologically Associated Environmental and Clinical Strains of Listeria monocytogenes by Random Amplification of Polymorphic DNA
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Updated: Jan 24, 2026

Listeria monocytogenes Infection of the Brain
Published on: October 2, 2018
Kevin G Kerr1, Peter Kite1, John Heritage1
1Department of Microbiology, University of Leeds, Old Medical School, Thoresby Place, Leeds, United Kingdom, LS2 9JT.
Abstract:
One hundred and three strains of Listeria monocytogenes (Lm), Listeria seeligeri and Listeria innocua of clinical, food, and environmental origin were examined by generating randomly amplified polymorphic DNA (RAPD). Using one 10 bp and two 13 bp random primers, epidemiologically related strains, previously shown to be indistinguishable by phage typing, yielded identical RAPD profiles. Strains isolated from the hands of three workers in a retail food establishment showed the presence of a single predominant Lm isolate. RAPD analysis is a rapid, reproducible, and relatively inexpensive method for the differentiation of epidemiologically linked isolates of Listeria spp.
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