Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Multiple Allele Traits01:49

Multiple Allele Traits

38.0K
The Concept of Multiple Allelism
38.0K
Multiple Regression01:25

Multiple Regression

3.8K
Multiple regression assesses a linear relationship between one response or dependent variable and two or more independent variables. It has many practical applications.
Farmers can use multiple regression to determine the crop yield based on more than one factor, such as water availability, fertilizer, soil properties, etc. Here, the crop yield is the response or dependent variable as it depends on the other independent variables. The analysis requires the construction of a scatter plot...
3.8K
Deformation of Member under Multiple Loadings01:11

Deformation of Member under Multiple Loadings

457
When a rod is made of different materials or has various cross-sections, it must be divided into parts that meet the necessary conditions for determining the deformation. These parts are each characterized by their internal force, cross-sectional area, length, and modulus of elasticity. These parameters are then used to compute the deformation of the entire rod.
In the case of a member with a variable cross-section, the strain is not constant but depends on the position. The deformation of an...
457
Multiple Voltage Sources01:25

Multiple Voltage Sources

1.7K
Generally, a single battery is not enough to power some devices. In such cases, batteries can be combined in two ways: in series or in parallel.
In series, the positive terminal of one battery is connected to the negative terminal of another battery. Hence, the voltage of each battery is added to give the net voltage, which is increased because each battery boosts the electrons that enter it. The same current flows through each battery because they are connected in series.
Batteries are...
1.7K
Multiple Comparison Tests01:13

Multiple Comparison Tests

4.4K
Multiple comparison test, abbreviated as MCT, is a post hoc analysis generally performed after comparing multiple samples with one or more tests. An MCT will help identify a significantly different sample among multiple samples or a factor among multiple factors.
It would be easy to compare two samples using a significance alpha level of 0.05. In other words, there is only one sample pair to be compared. However, it would be difficult to identify a significantly different sample if the number...
4.4K
Multiple Bar Graph01:07

Multiple Bar Graph

9.0K
As the name suggests, a multiple bar graph is the same as a bar graph but has multiple bars to depict relationships between different data values. One can include as many parameters as possible. However, each parameter must have the same unit of measurement.
Each bar or column in the multiple bar graph represents a data value. These graphs are used primarily in interrelating two or more sets of data. The categories of different kinds of data are listed along the horizontal or x-axis, whereas...
9.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SuperDecode: An integrated toolkit for analyzing mutations induced by genome editing.

Molecular plant·2025
Same author

<i>Phloeosinusmetasequoiae</i> sp. nov. (Coleoptera, Curculionidae, Scolytinae, Phloeosinini), a new insect pest of <i>Metasequoiaglyptostroboides</i> in China.

ZooKeys·2025
Same author

Exosomal miR‑3681‑3p from M2‑polarized macrophages confers cisplatin resistance to gastric cancer cells by targeting MLH1.

Molecular medicine reports·2025
Same author

Molecular evidence for goose astrovirus 2 and goose astrovirus 3 coinfection in goslings with gout.

Archives of virology·2025
Same author

Protein regulator of cytokinesis 1 regulates autophagy in hepatitis B virus‑associated liver cancer development.

Oncology reports·2025
Same author

Inhibition of Kv1.3 channel restrains macrophage M2 polarization and ameliorates renal fibrosis via regulating STAT6 phosphorylation.

Pharmacological research·2025

Related Experiment Video

Updated: Jan 24, 2026

Author Spotlight: Advancing Live-Cell Mechanobiology Through Fluorescence Microaspiration
07:02

Author Spotlight: Advancing Live-Cell Mechanobiology Through Fluorescence Microaspiration

Published on: January 12, 2024

1.5K

Robotic Micropipette Aspiration for Multiple Cells.

Yaowei Liu1, Maosheng Cui2, Jingjing Huang3

  • 1Institute of Robotics and Automatic Information System and the Tianjin Key Laboratory of Intelligent Robotics, Nankai University, Tianjin 300071, China. liuyaowei@mail.nankai.edu.cn.

Micromachines
|May 30, 2019
PubMed
Summary
This summary is machine-generated.

This study introduces a robotic system for rapid, automated cell elasticity measurement using micropipette aspiration (MA). The new method significantly increases efficiency for batch cell analysis, enabling optimized cell culturing protocols.

Keywords:
Young’s modulus measurementcell detectioncell transportationforce analysismicropipette aspirationrobotic batch cell manipulation

More Related Videos

Obtention of Giant Unilamellar Hybrid Vesicles by Electroformation and Measurement of their Mechanical Properties by Micropipette Aspiration
09:29

Obtention of Giant Unilamellar Hybrid Vesicles by Electroformation and Measurement of their Mechanical Properties by Micropipette Aspiration

Published on: January 19, 2020

9.0K
Robotics in Surgery: A Modular Robotic Platform Driven Gastric Wedge Resection
07:27

Robotics in Surgery: A Modular Robotic Platform Driven Gastric Wedge Resection

Published on: February 7, 2025

1.0K

Related Experiment Videos

Last Updated: Jan 24, 2026

Author Spotlight: Advancing Live-Cell Mechanobiology Through Fluorescence Microaspiration
07:02

Author Spotlight: Advancing Live-Cell Mechanobiology Through Fluorescence Microaspiration

Published on: January 12, 2024

1.5K
Obtention of Giant Unilamellar Hybrid Vesicles by Electroformation and Measurement of their Mechanical Properties by Micropipette Aspiration
09:29

Obtention of Giant Unilamellar Hybrid Vesicles by Electroformation and Measurement of their Mechanical Properties by Micropipette Aspiration

Published on: January 19, 2020

9.0K
Robotics in Surgery: A Modular Robotic Platform Driven Gastric Wedge Resection
07:27

Robotics in Surgery: A Modular Robotic Platform Driven Gastric Wedge Resection

Published on: February 7, 2025

1.0K

Area of Science:

  • Biophysics
  • Cell Biology
  • Robotics Engineering

Background:

  • Cell elasticity varies significantly between individual cells, necessitating batch measurements for statistical accuracy.
  • Current micropipette aspiration (MA) methods are inefficient for batch cell analysis due to limitations in cell storage and delivery.
  • Existing manual and robotic MA systems can only measure one cell at a time, hindering high-throughput studies.

Purpose of the Study:

  • To develop an automated robotic micropipette aspiration system for efficient batch cell elasticity measurement.
  • To overcome the low efficiency of current MA techniques by enabling simultaneous storage and sequential measurement of multiple cells.
  • To apply the developed system for optimizing pig oocyte maturation protocols through cell elasticity analysis.

Main Methods:

  • Development of a robotic MA system incorporating a feeder micropipette (FM) for cell storage and a measurement micropipette (MM) for aspiration.
  • Implementation of key techniques including determination of maximum permissible tilt angles for MM and FM, automated cell adhesion detection and breaking, and automated cell aspiration.
  • Integration of a multi-cell storage and sequential measurement capability.

Main Results:

  • The robotic system successfully measured the elasticity of over 20 cells continuously.
  • The manipulation speed was quadrupled compared to existing single-cell MA methods.
  • The system demonstrated the capability for efficient batch cell measurement, enabling analysis of pig oocyte elasticity under various culture conditions.

Conclusions:

  • The developed robotic MA system significantly enhances the efficiency of cell elasticity measurements for batch cell populations.
  • This advancement facilitates the statistical analysis of cell mechanical properties and the optimization of cell culture conditions.
  • The system provides a valuable tool for research in cell biology, biophysics, and reproductive science, particularly for improving oocyte maturation protocols.