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Microfluidic Applications for Disposable Diagnostics
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Diagnostic challenges in influenza.

Raúl Ortiz de Lejarazu Leonardo1, Silvia Rojo Rello1, Iván Sanz Muñoz2

  • 1Centro Nacional de Gripe de Valladolid, Universidad de Valladolid, Valladolid, España; Servicio de Microbiología e Inmunología, Hospital Clínico Universitario de Valladolid, Valladolid, España.

Enfermedades Infecciosas Y Microbiologia Clinica (English Ed.)
|May 30, 2019
PubMed
Summary

Many microbiology labs still don't detect influenza viruses, a cause of pneumonia. Implementing multiplex technologies can improve timely diagnosis and reduce antibiotic use.

Keywords:
CONSISEDiagnóstico de gripeEpidemiological surveillanceInfluenza diagnosisInfluenza virusNeuraminidasaNeuraminidaseSeroepidemiologySeroepidemiologíaVigilancia epidemiológicaVirus de la gripe

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Area of Science:

  • * Clinical virology and microbiology diagnostics.
  • * Infectious disease epidemiology and control.

Background:

  • * Influenza viruses remain significant etiological agents of community-acquired pneumonia.
  • * Subtyping and detection of influenza viruses are not universally performed in microbiology laboratories.
  • * Challenges persist in implementing advanced diagnostic technologies and resistance detection.

Purpose of the Study:

  • * To highlight the need for improved diagnostic capabilities for influenza and other respiratory viruses.
  • * To discuss the potential of multiplex technologies in clinical virology.
  • * To identify pending challenges in viral diagnostics, including severity markers and resistance detection.

Main Methods:

  • * Review of current practices in microbiology laboratories regarding influenza virus detection.
  • * Discussion of the advantages of molecular techniques over traditional viral culture.
  • * Exploration of future applications of sequencing and antibody detection techniques.

Main Results:

  • * Viral culture is being superseded by more sensitive and affordable molecular techniques for surveillance.
  • * Multiplex technologies offer enhanced feasibility for timely etiological diagnosis.
  • * Advanced techniques like sequencing and antibody detection are poised for future integration.

Conclusions:

  • * Widespread adoption of multiplex technologies is crucial for timely diagnosis of influenza and other respiratory viruses.
  • * Further development of diagnostic tools for severity markers and resistance is needed.
  • * Future strategies will integrate sequencing and antibody detection for comprehensive understanding of influenza epidemiology and vaccine development.