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Updated: Jan 24, 2026

Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking
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Triggering method in assisted reproduction alters the cumulus cell transcriptome.

Noga Fuchs Weizman1, Brandon A Wyse1, Itai Gat1

  • 1CReATe Fertility Centre, Toronto, ON, Canada.

Reproductive Biomedicine Online
|May 30, 2019
PubMed
Summary
This summary is machine-generated.

Dual triggering of final oocyte maturation enhances ovarian pathways and extracellular matrix remodeling. This method also reduces vascular growth and increases antioxidant protection for growing follicles in IVF cycles.

Keywords:
Blastulation rateCumulus–oocyte–complexDual triggerGnRH-agonist triggeringNext-generation sequencingTranscriptomic analysis

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Area of Science:

  • Reproductive biology
  • Genomics
  • In vitro fertilization (IVF)

Background:

  • The selection of the optimal method for triggering final oocyte maturation is crucial for successful IVF outcomes.
  • Understanding the molecular impact of different maturation-triggering strategies on cumulus cells is essential for optimizing fertility treatments.

Purpose of the Study:

  • To investigate the transcriptomic alterations in cumulus cells resulting from dual triggering compared to single triggering for final oocyte maturation.
  • To analyze these changes across different ovarian reserve statuses in patients undergoing IVF.

Main Methods:

  • A nested case-control study involving 60 patients undergoing gonadotrophin-releasing hormone antagonist (GnRH-ant) IVF cycles.
  • Stratification into high, normal, and low ovarian reserve subgroups.
  • Comparison of single trigger (human chorionic gonadotrophin [HCG] or gonadotrophin-releasing hormone agonist [GnRH-ag]) versus dual trigger (HCG + GnRH-ag) groups, with matched controls.
  • Next-generation sequencing of pooled cumulus cells from sibling oocytes.

Main Results:

  • Dual triggering significantly supports key ovarian pathways involved in oocyte maturation.
  • Dual triggering promotes extracellular matrix remodeling processes.
  • Attenuated vasculo-endothelial growth and enhanced antioxidant protection were observed in the dual trigger group.

Conclusions:

  • This study provides the first transcriptomic insights into dual triggering for final oocyte maturation across diverse patient populations.
  • Findings highlight the necessity for larger studies to validate the transcriptomic effects of oocyte maturation triggering methods.
  • Further large-scale randomized controlled trials are needed to correlate these transcriptomic findings with clinical outcomes in IVF.