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Related Experiment Videos

The immunoglobulin lambda locus in rat consists of two C lambda genes and a single V lambda gene.

M L Steen, L Hellman, U Pettersson

    Gene
    |January 1, 1987
    PubMed
    Summary

    The rat immunoglobulin lambda locus features a single V lambda gene and two C lambda genes, with one C lambda likely a pseudogene. Its organization is similar to the mouse but with a single cluster, suggesting evolutionary divergence.

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    Area of Science:

    • Immunogenetics
    • Comparative Genomics

    Background:

    • The immunoglobulin lambda (V lambda) light chain locus is crucial for adaptive immunity.
    • Understanding V lambda locus organization across species provides insights into immune system evolution.

    Purpose of the Study:

    • To characterize the germline V lambda and C lambda genes in the rat.
    • To compare the rat V lambda locus organization with that of other species, particularly the mouse.

    Main Methods:

    • Isolation of germline V lambda and C lambda genes using recombinant-phage libraries.
    • Nucleotide sequencing for gene characterization and analysis of regulatory elements.

    Main Results:

    • The rat lambda locus contains a single V lambda gene and two C lambda genes, making it one of the simplest characterized.

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  • Two J segments are located 5' to each C lambda gene; one C lambda gene (C lambda 1) appears to be a pseudogene due to non-functional signals.
  • The locus organization resembles the mouse but with a single cluster, unlike the mouse's multiple clusters.

    • Conclusions:

    • The rat V lambda locus is highly simplified compared to other species.
    • Evolutionary divergence between rats and mice (10 million years ago) may have led to gene loss in rats or duplication in mice.