Contralateral fatigue during severe-intensity single-leg exercise: influence of acute acetaminophen ingestion

Exhaustive single-leg exercise has been suggested to reduce time to task failure (T_lim) during subsequent exercise in the contralateral leg by exacerbating central fatigue development. We investigated the influence of acetaminophen (ACT), an analgesic that may blunt central fatigue development, on T_lim during single-leg exercise completed with and without prior fatiguing exercise of the contralateral leg. Fourteen recreationally active men performed single-leg severe-intensity knee-extensor exercise to T_lim on the left (Leg₁) and right (Leg₂) legs without prior contralateral fatigue and on Leg₂ immediately following Leg₁ (Leg_2-CONTRA). The tests were completed following ingestion of 1-g ACT or maltodextrin [placebo (PL)] capsules. Intramuscular phosphorus-containing metabolites and substrates and muscle activation were assessed using ³¹P-MRS and electromyography, respectively. T_lim was not different between Leg_1ACT and Leg_1PL conditions (402 ± 101 vs. 390 ± 106 s, P = 0.11). There was also no difference in T_lim between Leg_2ACT-CONTRA and Leg_2PL-CONTRA (324 ± 85 vs. 311 ± 92 s, P = 0.10), but T_lim was shorter in Leg_2ACT-CONTRA and Leg_2PL-CONTRA than in Leg_2CON (385 ± 104 s, both P < 0.05). There were no differences in intramuscular phosphorus-containing metabolites and substrates or muscle activation between Leg_1ACT and Leg_1PL and between Leg_2ACT-CONTRA and Leg_2PL-CONTRA (all P > 0.05). These findings suggest that levels of metabolic perturbation and muscle activation at T_lim are not different during single-leg severe-intensity knee-extensor exercise completed with or without prior fatiguing exercise of the contralateral leg. Despite contralateral fatigue, ACT ingestion did not alter neuromuscular responses, muscle metabolites, or exercise performance.