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Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance
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Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

Published on: May 2, 2018

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Multiple Antibiotic Resistance (MAR), Plasmid Profiles, and DNA Polymorphisms among Vibrio vulnificus Isolates

Mohammed M Kurdi Al-Dulaimi1, Sahilah Abd Mutalib2, Maaruf Abd Ghani3

  • 1Education Supervision Department, Office of Assistant undersecretary for Educational Affairs, Ministry of Education and Higher Education, Doha, Qatar. bio_micro1978@yahoo.co.uk.

Antibiotics (Basel, Switzerland)
|May 31, 2019
PubMed

Insights

Vibrio vulnificus strains from Malaysian cockles and Qatari clams show high multiple antibiotic resistance (MAR) and genetic diversity. This poses a significant risk to human health from consuming contaminated seafood.

Area of Science:

  • Microbiology
  • Environmental Science
  • Public Health

Background:

  • Vibrio vulnificus is a significant foodborne pathogen.
  • Antibiotic resistance in V. vulnificus is a growing global concern.
  • Seafood consumption is a primary route of V. vulnificus transmission.

Purpose of the Study:

  • To assess the multiple antibiotic resistance (MAR) index of V. vulnificus.
  • To analyze plasmid profiles and DNA polymorphisms in V. vulnificus strains.
  • To evaluate the public health implications of V. vulnificus in seafood.

Main Methods:

  • Sixty V. vulnificus strains were isolated from cockles (Malaysia) and clams (Qatar).
  • Multiple antibiotic resistance (MAR) index, plasmid profiles, and DNA polymorphisms were analyzed.
  • Phylogenetic analysis was performed using GelCompar II software.

Main Results:

  • All isolates exhibited resistance to at least two antibiotics, with high resistance to penicillin, ampicillin, cephalothin, clindamycin, vancomycin, and erythromycin.
  • MAR index values ranged from 0.2 to 0.7, indicating significant antibiotic resistance.
  • Sixty-five percent of strains harbored plasmids, and phylogenetic analysis revealed genomic heterogeneity.

Conclusions:

  • V. vulnificus strains from Malaysian and Qatari seafood display high MAR and genomic diversity.
  • The findings highlight a significant public health concern for consumers of contaminated shellfish.
  • Effective monitoring and control strategies are crucial to mitigate risks associated with V. vulnificus.
Keywords:
DNA polymorphismsVibrio vulnificusgenomic heterogeneitymultiple antibiotic resistance (MAR) indexplasmid profiles

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