The imbalance between regulatory memory B cells reveals possible pathogenesis involvement in pediatric immune...

Introduction: CD19⁺CD24^hiCD38^hi regulatory B cells (B_regs) and CD19⁺CD27⁺ memory B cells (B_mems) are B cell subsets with specific immunoregulatory properties. In this study, the balance of these subsets was investigated in pediatric immune thrombocytopenia (ITP) patients, and the frequencies of B_regs and B_mems before and after first-line therapy were measured. Methods: Forty-nine pediatric ITP patients and 19 normal controls were enrolled in this study. The total CD19⁺ B cells, B_regs and B_mems in the peripheral blood (PB) of all cases were measured by flow cytometry. Results: We found higher frequencies of total CD19⁺ B cells and B_mems in newly diagnosed ITP patients than those in normal controls (p < 0.01), whereas the frequencies of CD19⁺CD24^hiCD38^hi B_regs was significantly lower in ITP patients (p < 0.001). After therapy with MP + IVIG, the level of CD19⁺CD24^hiCD38^hi B_regs and B_mems were almost normalized. Conclusion: Our results indicated that pediatric ITP patients were characterized by a decline in CD19⁺CD24^hiCD38^hi Bregs and increment of CD19⁺CD27⁺Bmems, and an increase of total CD19⁺ B cells in their peripheral blood.