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DisV-HPV16, versatile and powerful software to detect HPV in RNA sequencing data
Bingqing Yan1,2, Xinyi Liu1,2, Siwei Zhang1,2
1College of Basic Medicine, Harbin Medical University, Harbin, 150081, China.
BMC Infectious Diseases
|May 31, 2019
Summary
DisV-HPV16 is new software that rapidly and reliably detects Human Papillomavirus type 16 (HPV16) and its oncogene expression from RNA sequencing data, aiding cancer research.
Area of Science:
- Genomics
- Virology
- Bioinformatics
Background:
- High-throughput sequencing data enables investigation of viral elements in host genomes linked to cancer.
- Current tools primarily detect viral infection or integration, not oncogene expression.
- Human Papillomavirus type 16 (HPV16) oncogene expression is crucial in carcinoma development.
Purpose of the Study:
- To develop novel software, DisV-HPV16, for evaluating HPV16 oncogene expression.
- To provide a tool for analyzing viral oncogene expression in host genomes.
Main Methods:
- Development of DisV-HPV16 software.
- Analysis of RNA sequencing data.
- Modification of reference files for enhanced virus detection.
Main Results:
- DisV-HPV16 detected HPV16 virus and oncogene expression faster than existing software.
- The software demonstrated high convenience in detecting viruses after reference file modification.
- Empirical laboratory experiments confirmed DisV-HPV16's accuracy and reliability.
Conclusions:
- DisV-HPV16 is a dependable software for detecting viruses and viral oncogene expression using RNA sequencing data.
- This tool offers deeper insights into viral infection status and gene expression.
- DisV-HPV16 enhances the analysis of viral and host cell gene expression.
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