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Anti-CD3 activation of T cells with immunoglobulin coupled red cells
We have studied the ability of anti-mouse IgG antibody coupled to Burro red blood cells (BRBC) to modulate the anti-CD3 stimulation of human T cells. These coupled BRBC restored anti-Leu-4 (anti-CD3) induced proliferative responses to purified T cells, in the presence of interleukin-1 (IL-1). Uncoupled BRBC were incapable of supporting anti-Leu-4-induced stimulation. T cells from individuals whose peripheral blood mononuclear cells were unresponsive to anti-Leu-4 did proliferative in the presence of anti-Leu-4, IL-1, and coupled BRBC. Ig-coupled red cells provide an efficient method for the study of murine antibody-induced modulation of T cell surface antigens.
We have studied the ability of anti-mouse IgG antibody coupled to Burro red blood cells (BRBC) to modulate the anti-CD3 stimulation of human T cells. These coupled BRBC restored anti-Leu-4 (anti-CD3) induced proliferative responses to purified T cells, in the presence of interleukin-1 (IL-1). Uncoupled BRBC were incapable of supporting anti-Leu-4-induced stimulation. T cells from individuals whose peripheral blood mononuclear cells were unresponsive to anti-Leu-4 did proliferative in the presence of anti-Leu-4, IL-1, and coupled BRBC. Ig-coupled red cells provide an efficient method for the study of murine antibody-induced modulation of T cell surface antigens.