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MSCs inhibits the angiogenesis of HUVECs through the miR-211/Prox1 pathway

Jian Pan1, Xianglong Wang1, Dequan Li2

  • 1Department of Ophthalmology, The First Affiliated Hospital of Wenzhou Medical University, Nanbaixiang, Ouhai District, Wenzhou, Zhejiang, China.

Journal of Biochemistry
|May 31, 2019
PubMed

Insights

Mesenchymal stem cells (MSCs) reduce corneal neovascularization and opacity by inhibiting human umbilical vein endothelial cells (HUVECs) angiogenesis. This effect is mediated by miR-211, which down-regulates Prox1 expression.

Area of Science:

  • Ophthalmology
  • Regenerative Medicine
  • Molecular Biology

Background:

  • Corneal neovascularization (CNV) is a significant cause of vision impairment.
  • Mesenchymal stem cells (MSCs) show therapeutic potential in ocular surface diseases.
  • Understanding the molecular mechanisms of MSCs in controlling angiogenesis is crucial.

Purpose of the Study:

  • To investigate the effect of MSCs on angiogenesis of human umbilical vein endothelial cells (HUVECs).
  • To elucidate the role of miR-211 and Prox1 in MSC-mediated inhibition of corneal angiogenesis.

Main Methods:

  • Subconjunctival injection of MSCs in a rat corneal alkali burn model.
  • Evaluation of corneal neovascularization (CNV) and opacity.
  • In vitro tube formation assay using HUVECs.
  • Assessment of miR-211 and Prox1 expression levels.

Main Results:

  • MSCs significantly reduced CNV and corneal opacity in rats.
  • MSC treatment inhibited HUVECs angiogenesis in vitro.
  • miR-211 overexpression suppressed HUVECs angiogenesis by down-regulating Prox1.
  • Knockdown of miR-211 reversed the inhibitory effects of MSCs on HUVECs angiogenesis and Prox1 expression.

Conclusions:

  • MSCs inhibit HUVECs angiogenesis through the miR-211/Prox1 pathway.
  • miR-211 plays a critical role in MSC-mediated inhibition of corneal angiogenesis.
  • This study reveals a novel mechanism for MSCs in treating corneal neovascularization.
Keywords:
HUVECsMSCsProx1corneal angiogenesismiR-211

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