Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nuclear Magnetic Resonance (NMR): Overview01:07

Nuclear Magnetic Resonance (NMR): Overview

6.8K
Nuclear magnetic resonance (NMR) is a phenomenon exhibited by certain nuclei that can absorb characteristic radio frequency radiation under certain conditions. NMR has been extensively applied in molecular spectroscopy and medical diagnostic imaging. In both these applications, the molecule or subject under study is placed in a magnetic field and irradiated with radio frequency energy.
NMR spectroscopy generates a spectrum where the characteristic absorption frequencies of the sample are...
6.8K
Magnetic Resonance Imaging01:24

Magnetic Resonance Imaging

9.2K
Magnetic resonance imaging (MRI) is a noninvasive medical imaging technique based on a phenomenon of nuclear physics discovered in the 1930s, in which matter exposed to magnetic fields and radio waves was found to emit radio signals. In 1970, a physician and researcher named Raymond Damadian noticed that malignant (cancerous) tissue gave off different signals than normal body tissue. He applied for a patent for the first MRI scanning device in clinical use by the early 1980s. The early MRI...
9.2K
Atomic Nuclei: Magnetic Resonance01:05

Atomic Nuclei: Magnetic Resonance

1.2K
The number of nuclear spins aligned in the lower energy state is slightly greater than those in the higher energy state. In the presence of an external magnetic field, as the spins precess at the Larmor frequency, the excess population results in a net magnetization oriented along the z axis. When a pulse or a short burst of radio waves at the Larmor frequency is applied along the x axis, the coupling of frequencies causes resonance and flips the nuclear spins of the excess population from the...
1.2K
Atomic Nuclei: Nuclear Magnetic Moment00:59

Atomic Nuclei: Nuclear Magnetic Moment

3.2K
All atomic nuclei are positively charged. When they have a nonzero spin, they behave like rotating charges. As a consequence of their charge and spin, these nuclei generate a magnetic field (B). This, in turn, gives rise to a magnetic moment (μ), which is randomly oriented in the absence of an external magnetic field. When an external magnetic field (B0) is applied, the magnetic moment vectors can align with the field or against it in 2 + 1 orientations. A hydrogen nucleus, which is just a...
3.2K
Nuclear Fusion02:45

Nuclear Fusion

33.7K
The process of converting very light nuclei into heavier nuclei is also accompanied by the conversion of mass into large amounts of energy, a process called fusion. The principal source of energy in the sun is a net fusion reaction in which four hydrogen nuclei fuse and ultimately produce one helium nucleus and two positrons.
A helium nucleus has a mass that is 0.7% less than that of four hydrogen nuclei; this lost mass is converted into energy during the fusion. This reaction produces about...
33.7K
Nuclear Stability03:18

Nuclear Stability

23.0K
Protons and neutrons, collectively called nucleons, are packed together tightly in a nucleus. With a radius of about 10−15 meters, a nucleus is quite small compared to the radius of the entire atom, which is about 10−10 meters. Nuclei are extremely dense compared to bulk matter, averaging 1.8 × 1014 grams per cubic centimeter. If the earth’s density were equal to the average nuclear density, the earth’s radius would be only about 200 meters.
To hold positively charged protons together...
23.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synthesis of N-Acetyl-D- and -L-Leucine-<sup>13</sup>C<sub>6</sub> Tool Compounds in Neurodegenerative Disease.

ChemMedChem·2026
Same author

Improved SABRE hyperpolarisation using pulse sequences to reduce effective coupling.

Physical chemistry chemical physics : PCCP·2026
Same author

Gas-phase spectroscopy of H<sub>2</sub>O@C<sub>60</sub><sup>+</sup> and H<sub>2</sub>O@C<sub>60</sub>H<sup>+</sup> in the mid-infrared: the challenges of searching for endohedral fullerenes in space.

Physical chemistry chemical physics : PCCP·2026
Same author

Error Compensation without a Time Penalty: Robust Spin-Lock-Induced Crossing in Solution NMR.

The journal of physical chemistry letters·2026
Same author

Zero- to ultralow-field J-spectroscopy with a diamond magnetometer.

Communications chemistry·2026
Same author

Microfluidic NMR for <i>operando</i> monitoring of drug-induced metabolic fluxes in liver tissue slices.

Lab on a chip·2026

Related Experiment Video

Updated: Jan 24, 2026

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

11.0K

High-Resolution Nuclear Magnetic Resonance Spectroscopy with Picomole Sensitivity by Hyperpolarization on a Chip.

James Eills1, William Hale1, Manvendra Sharma1

  • 1School of Chemistry , University of Southampton , Southampton , Hampshire SO17 1BJ , United Kingdom.

Journal of the American Chemical Society
|May 31, 2019
PubMed
Summary

High-resolution NMR achieved picomole sensitivity for micromolar analytes using parahydrogen-induced hyperpolarization (PHIP) and microdetectors. This breakthrough enables sensitive detection in microfluidic devices, overcoming previous concentration limitations.

More Related Videos

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy
07:02

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy

Published on: December 16, 2021

1.8K
Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts
09:01

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts

Published on: September 21, 2014

15.2K

Related Experiment Videos

Last Updated: Jan 24, 2026

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

11.0K
Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy
07:02

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy

Published on: December 16, 2021

1.8K
Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts
09:01

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts

Published on: September 21, 2014

15.2K

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Microfluidics

Background:

  • Microfluidic NMR systems offer high mass sensitivity due to small sample volumes.
  • Detecting analytes at less than millimolar concentrations in microfluidic NMR is challenging.
  • Parahydrogen-induced hyperpolarization (PHIP) enhances NMR signal intensity.

Purpose of the Study:

  • To achieve picomole sensitivity for micromolar analyte concentrations in microfluidic NMR.
  • To overcome the concentration limitations of current microfluidic NMR systems.
  • To integrate PHIP with microfluidic NMR for enhanced detection capabilities.

Main Methods:

  • Implementation of parahydrogen-induced hyperpolarization (PHIP) on a microfluidic chip.
  • Utilizing a high-sensitivity transmission line microdetector with a 2.5 μL detection volume.
  • Introducing para-enriched hydrogen gas via membrane diffusion into the solution.

Main Results:

  • Achieved picomole sensitivity for micromolar concentrations, a limit of detection better than .
  • Minimized polarization losses by integrating the hydrogenation reaction within the microfluidic chip.
  • Demonstrated stable and sensitive system performance, allowing quantitative analysis and 2D NMR experiments.

Conclusions:

  • The developed PHIP-enhanced microfluidic NMR system significantly enhances sensitivity for low-concentration analytes.
  • This approach overcomes a critical limitation in microfluidic NMR, enabling detection at unprecedentedly low levels.
  • The system is suitable for quantitative analysis and advanced NMR techniques like homo- and heteronuclear 2D NMR.