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Updated: Jan 24, 2026

Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification
Published on: November 28, 2016
Human high-density lipoprotein microtranscriptome is unique and suggests an extended role in lipid metabolism
Véronique Desgagné1,2, Renée Guérin1,3, Simon-Pierre Guay1,2,4,5
1Department of Biochemistry, Université de Sherbrooke, Sherbrooke, Québec, J1H 5N4, Canada.
Abstract:
Aim: To comprehensively characterize the high-density lipoproteins (HDLs) microtranscriptome and to assess whether it is distinct from that of plasma and different between women and men. Methods: RNA was extracted from ultracentrifugation-purified HDLs and plasma from 17 healthy women and men couples, and libraries were sequenced on a HiSeq2500 platform. Results: On average, 310 ± 64 and 355 ± 31 miRNAs were detected (≥1 read per million) in HDLs and plasma, respectively. A total of 62 and 134 miRNAs were over-represented (e.g., miR-150-5p; fold change = 7.52; padj = 5.41 × 10-111) and under-represented (e.g., miR-22-3p; fold change = -5.28; padj = 2.11 × 10-154) in HDLs compared with plasma. These miRNAs were enriched in lipid metabolism and cellular processes-related pathways. Conclusion: HDLs exhibit a sex-independent miRNA profile distinct from that of plasma. These miRNAs may contribute to the HDLs' physiology.
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