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Related Experiment Videos

Cell surface oligosaccharide modulation during differentiation. I. Modulation of lectin binding.

P L Mann, I Lopez-Colberg, R O Kelley

    Mechanisms of Ageing and Development
    |May 1, 1987
    PubMed
    Summary

    Cellular senescence involves dynamic changes in cell surface sugar structures (oligosaccharides). These alterations in oligosaccharide expression occur before visible signs of aging in human lung fibroblasts, impacting cell growth control.

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    Area of Science:

    • Cell Biology
    • Glycobiology
    • Biochemistry

    Background:

    • Cell surface oligosaccharides play crucial roles in cellular processes.
    • Understanding changes in cell surface carbohydrates is vital for comprehending cell aging and growth regulation.

    Purpose of the Study:

    • To investigate quantitative and qualitative changes in cell surface oligosaccharide expression in IMR-90 human lung fibroblasts during their lifespan.
    • To correlate these changes with growth control and cellular senescence.

    Main Methods:

    • Utilized FITC-conjugated lectins (Concanavalin-A, Wheat Germ Agglutinin, Ricinus communis agglutinin, Dolichos biflorus agglutinin) for single-cell analysis.
    • Employed a biotin-avidin-enzyme amplification assay for population-level quantitation.
    • Studied randomly growing cultures across different population doubling levels (PDL).

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    Main Results:

    • Observed a 20-40% decrease in binding for Concanavalin-A, Wheat Germ Agglutinin, and Ricinus communis agglutinin as cells aged.
    • Documented a 100% increase in Dolichos biflorus agglutinin binding over the same period.
    • Detected significant oligosaccharide changes prior to the onset of morphological senescence markers (around PDL 45).

    Conclusions:

    • Cell surface oligosaccharide expression is dynamic and changes significantly with fibroblast aging.
    • These alterations precede overt signs of senescence and suggest a role in growth control.
    • Oligosaccharide modifications are integral to cellular senescence and growth regulation mechanisms.