Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Anchoring-and-Adjustment Heuristic01:25

The Anchoring-and-Adjustment Heuristic

7.7K
In order to make good decisions, we use our knowledge and our reasoning. Often, this knowledge and reasoning is sound and solid. However, sometimes, we are swayed by biases or by others manipulating a situation. For example, let’s say you and three friends wanted to rent a house and had a combined target budget of $1,600. The realtor shows you only very run-down houses for $1,600 and then shows you a very nice house for $2,000. Might you ask each person to pay more in rent to get the...
7.7K
Lipids as Anchors01:32

Lipids as Anchors

7.2K
In the plasma membrane, the lipids forming the bilayer can also act as an anchor to tether proteins to the membrane. The three main types of lipid anchors found in eukaryotes are – prenyl groups, fatty acyl groups, and glycosylphosphatidylinositol or GPI groups. Prenyl and fatty acyl groups act as anchors on the cytosolic surface of the membrane, whereas GPI anchors proteins on the extracellular side.
The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains...
7.2K
Anchoring Junctions01:03

Anchoring Junctions

4.9K
Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
4.9K
GPI Anchoring of Proteins in the ER Membrane01:29

GPI Anchoring of Proteins in the ER Membrane

5.4K
GPI-anchoring is a post-translational, reversible protein modification that is ubiquitous in eukaryotes. Such proteins are primarily present on the exoplasmic leaflet of the plasma membrane.
GPI-anchor structure
A sequence of 11 enzymatic reactions results in the synthesis of the complete GPI anchor consisting of a hydrophobic and a hydrophilic portion. The hydrophobic portion comprises phosphatidylinositol, while the hydrophilic part comprises polar groups like phosphoethanolamine,...
5.4K
Tail-anchoring of Proteins in the ER Membrane01:45

Tail-anchoring of Proteins in the ER Membrane

3.7K
Tail-anchored, or TA, proteins are estimated to make up to 3-5% of membrane proteins found in the eukaryotic cell. Such proteins have a single transmembrane domain located approximately 30 amino acid residues upstream from the C-terminal end. As a result, the signal recognition particle (SRP) cannot guide a TA protein to the ER membrane for cotranslational insertion. Hence, they are integrated into the ER membrane post-translationally using their C-terminal end as the anchor. TA proteins...
3.7K
Naturalistic Observations02:30

Naturalistic Observations

17.0K
If you want to understand how behavior occurs, one of the best ways to gain information is to simply observe the behavior in its natural context. However, people might change their behavior in unexpected ways if they know they are being observed. How do researchers obtain accurate information when people tend to hide their natural behavior? As an example, imagine that your professor asks everyone in your class to raise their hand if they always wash their hands after using the restroom. Chances...
17.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multicellular sensor arrays fabricated by capillary stamping for pattern-based odor discrimination.

Lab on a chip·2026
Same author

Open-air human skin equivalent platform enabling photobiological studies and topical product testing.

Biofabrication·2026
Same author

Cell-Based Multisensor Array for Vapor-Phase Detection of Cancer-Related Compounds in Human Urine.

ACS sensors·2026
Same author

Prevascularized grafts with spatially organized MSC spheroids to accelerate therapeutic angiogenesis in ischemic disease.

Angiogenesis·2026
Same author

Longitudinal tracking of dyadic body temperature synchrony in social pairs across species.

Scientific reports·2026
Same author

Load-programmable training platform for load-response characterization of engineered skeletal muscle tissue.

Biofabrication·2026

Related Experiment Video

Updated: Jan 24, 2026

Robust Differentiation of Human iPSCs into a Pure Population of Adipocytes to Study Adipocyte-Associated Disorders
10:31

Robust Differentiation of Human iPSCs into a Pure Population of Adipocytes to Study Adipocyte-Associated Disorders

Published on: February 9, 2022

4.1K

Temporal Observation of Adipocyte Microfiber Using Anchoring Device.

Akiyo Yokomizo1, Yuya Morimoto2,3, Keigo Nishimura4,5

  • 1Center for International Research on Integrative Biomedical Systems (CIBiS), Institute of Industrial Science (IIS), The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan. yokomizo@iis.u-tokyo.ac.jp.

Micromachines
|June 1, 2019
PubMed
Summary

Researchers developed an anchoring device to immobilize adipocyte microfibers for long-term study. This tool enables tracking and analysis of 3D-cultured fat cells and their response to anti-obesity drugs.

Keywords:
adipose tissuebiofabricationlipolysismicrofluidics

More Related Videos

Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures MAAC
06:28

Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures MAAC

Published on: February 13, 2020

20.3K
An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function
09:20

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function

Published on: May 4, 2021

4.2K

Related Experiment Videos

Last Updated: Jan 24, 2026

Robust Differentiation of Human iPSCs into a Pure Population of Adipocytes to Study Adipocyte-Associated Disorders
10:31

Robust Differentiation of Human iPSCs into a Pure Population of Adipocytes to Study Adipocyte-Associated Disorders

Published on: February 9, 2022

4.1K
Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures MAAC
06:28

Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures MAAC

Published on: February 13, 2020

20.3K
An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function
09:20

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function

Published on: May 4, 2021

4.2K

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Tissue Engineering

Background:

  • Adipocyte microfibers are crucial for studying fat tissue.
  • Long-term observation of these microfibers is challenging due to culture medium manipulation and transport.
  • A stable method for immobilizing adipocyte microfibers is needed for extended functional and morphological analysis.

Purpose of the Study:

  • To develop and validate an anchoring device for immobilizing adipocyte microfibers.
  • To enable long-term temporal observations of three-dimensional cultured adipocytes.
  • To assess the utility of the device for drug testing, specifically evaluating lipolysis.

Main Methods:

  • An anchoring device with pillars was designed to immobilize alginate-encased adipocyte microfibers.
  • The device facilitated stable microfiber positioning during culture medium exchange and transportation.
  • Temporal observations of adipocyte morphology and function were conducted over one month.
  • Lipolysis assays were performed using anti-obesity reagents to test drug efficacy.

Main Results:

  • The anchoring device successfully immobilized adipocyte microfibers, allowing for stable, long-term culture.
  • Researchers achieved continuous monitoring of three-dimensional cultured adipocytes for a month.
  • The device demonstrated applicability in drug testing by evaluating adipocyte lipolysis in response to therapeutic agents.

Conclusions:

  • The proposed anchoring device provides a stable platform for long-term observation of adipocyte microfibers.
  • This technology facilitates the study of adipocyte function and morphology in a 3D-cultured environment.
  • The device is a promising tool for temporal biochemical analyses and drug screening, particularly for anti-obesity research.