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Long noncoding RNA FAM3D-AS1 inhibits development of colorectal cancer through NF-κB signaling pathway

Ying Meng1, Feng Yu2

  • 1The First Division of Cancer Department of Zibo Central Hospital, Shandong, China.

Bioscience Reports
|June 1, 2019
PubMed

Insights

Long noncoding RNA FAM3D-AS1 inhibits colorectal cancer (CRC) progression by suppressing cell proliferation and invasion. This study reveals FAM3D-AS1

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • The role of long noncoding RNAs (lncRNAs) in colorectal cancer (CRC) remains largely undefined.
  • Identifying novel therapeutic targets for CRC is crucial for improving patient outcomes.

Purpose of the Study:

  • To investigate the function of lncRNA FAM3D-AS1 in colorectal cancer.
  • To elucidate the underlying molecular mechanism of FAM3D-AS1 in CRC development.

Main Methods:

  • Cell proliferation, invasion, and death assays (CCK8, Transwell, trypan blue staining).
  • Quantitative real-time PCR to determine FAM3D-AS1 expression levels.
  • Analysis of epithelial-mesenchymal transition (EMT) and NF-κB signaling pathway.

Main Results:

  • lncRNA FAM3D-AS1 was found to be located in the cytoplasm.
  • Overexpression of FAM3D-AS1 significantly inhibited CRC cell proliferation, survival, and invasion.
  • FAM3D-AS1 was demonstrated to reverse the EMT process and inhibit CRC via the NF-κB signaling pathway.

Conclusions:

  • lncRNA FAM3D-AS1 plays a tumor-suppressive role in colorectal cancer.
  • FAM3D-AS1 inhibits CRC development by targeting the NF-κB signaling pathway.
  • FAM3D-AS1 represents a potential novel therapeutic target for colorectal cancer treatment.
Keywords:
Colorectal cancerEMTlncRNA

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