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Updated: Jan 24, 2026

Visualization of Biofilm Formation in Candida albicans Using an Automated Microfluidic Device
Published on: December 14, 2017
A novel anti Candida albicans drug screening system based on high-throughput microfluidic chips
Le Qiang1, Jing Guo2, Yingkuan Han1
1Institute of Marine Science and Technology, Shandong University, 72 Binhai Road, Qingdao, 266237, China.
Abstract:
Due to the antibacterial resistance crisis, developing new antibacterials is of particular interest. In this study, we combined the antifungal drug amphotericin B with 50,520 different small molecule compounds obtained from the Chinese National Compound Library in an attempt to improve its efficacy against Candida albicans persister cells. To systematically study the antifungal effect of each compound, we utilized custom-designed high-throughput microfluidic chips. Our microfluidic chips contained microchannels ranging from 3 µm to 5 µm in width to allow Candida albicans cells to line up one-by-one to facilitate fluorescence-microscope viewing. After screening, we were left with 10 small molecule compounds that improved the antifungal effects of amphotericin B more than 30% against Candida albicans persister cells.
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