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Updated: Jan 24, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

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hnRNPC regulates cancer-specific alternative cleavage and polyadenylation profiles

Harry Fischl1, Jonathan Neve1, Zhiqiao Wang1

  • 1Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK.

Nucleic Acids Research
|June 1, 2019
PubMed

Insights

Alternative cleavage and polyadenylation (APA) generates distinct mRNA profiles in colon cancer progression. Overexpression of hnRNPC drives metastatic cancer cell APA profiles, impacting cancer-related genes like MTHFD1L.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • Alternative cleavage and polyadenylation (APA) expands the mRNA isoform repertoire, influencing gene expression.
  • Deregulation of APA is linked to diseases, including cancer progression, but its role in cancer-specific isoform generation is unclear.

Purpose of the Study:

  • To investigate how APA generates cancer-specific isoform profiles during colon cancer progression.
  • To determine the physiological consequences of these APA profiles.

Main Methods:

  • Utilized a subcellular fractionation approach.
  • Analyzed nuclear and cytoplasmic APA profiles across successive stages of colon cancer using a cell line model.

Main Results:

  • Demonstrated the establishment of specific APA profiles during cancer progression.
  • Identified hnRNPC overexpression as critical for APA profiles in metastatic colon cancer cells.
  • Showed hnRNPC regulates poly(A) site selection in cancer-associated genes, including MTHFD1L.

Conclusions:

  • APA plays a significant role in shaping gene expression during colon cancer progression.
  • hnRNPC is a key regulator of APA in metastatic colon cancer, affecting genes vital for cancer development.

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