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Updated: Jan 24, 2026

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
Published on: October 15, 2021
Peptide Trimming for MHC Class I Presentation by Endoplasmic Reticulum Aminopeptidases
Mirco Compagnone1, Doriana Fruci2
1Immuno-Oncology Laboratory, Department of Pediatric Onco-Hematology, Cell and Gene Therapy, Bambino Gesù Children's Hospital-IRCCS, Rome, Italy.
Abstract:
Endoplasmic reticulum aminopeptidases ERAP1 and ERAP2 have recently emerged as important players in regulating innate and adaptive immune responses by trimming peptide ligands for MHC class I molecules. Functional polymorphisms in ERAP1 and ERAP2 genes have been associated with predisposition to several diseases including autoimmune diseases, viral infections, and virally induced cancers. In this chapter, we describe two basic methods for monitoring peptide-trimming activity by ER aminopeptidases and screening potential chemical inhibitors.
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