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Published on: September 17, 2008
Testing the Impact of Protease Inhibitors in Antigen Presentation Assays
Michael Basler1,2, Marcus Groettrup3,4
1Biotechnology Institute Thurgau at the University of Konstanz, Kreuzlingen, Switzerland. michael.basler@uni-konstanz.de.
Abstract:
The major histocompatibility complex (MHC) class I restricted pathway of antigen processing allows the presentation of intracellular antigens to cytotoxic T lymphocytes. The proteasome is the main protease in the cytoplasm and the nucleus, which is responsible for the generation of most peptide ligands of MHC-I molecules. Peptides produced by the proteasome can be further trimmed or destroyed by numerous cytosolic or endoplasmic reticulum (ER) luminal proteases. Small molecule inhibitors are useful tools for probing the role of proteases in MHC class I antigen processing. Here, we describe different methods to test the impact of protease inhibitors in antigen presentation assays.
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