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Updated: Jan 24, 2026

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
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Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

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Purification and Identification of Naturally Presented MHC Class I and II Ligands

Annika Nelde1, Daniel J Kowalewski1, Stefan Stevanović2

  • 1Department of Immunology, Interfaculty Institute for Cell Biology, University of Tübingen, Tübingen, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|June 1, 2019
PubMed

The large-scale and in-depth identification of MHC class I- and II-presented peptides is indispensable for gaining insight into the fundamental rules of immune recognition as well as for developing innovative immunotherapeutic approaches against cancer and other diseases. In this chapter we briefly review the existing strategies for the isolation of MHC-restricted peptides and provide a detailed protocol for the immunoaffinity purification of MHC class I- and II-presented peptides from primary tissues or cells.

Keywords:
Antigen presentationHuman leukocyte antigen (HLA)Immunoaffinity purificationImmunopeptidomicsLigandome analysisMajor histocompatibility complex (MHC)Mass spectrometry

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