Identification of MHC Ligands and Establishing MHC Class I Peptide Motifs

MHC class I peptide motifs are used on a regular basis to identify and predict MHC class I ligands and CD8⁺ T cell epitopes. This approach is above all an invaluable tool for the identification of disease-associated epitopes ranging from pathogen associated epitopes, tumor associated natural and neoepitopes to autoimmune disease associated epitopes. As a matter of fact, the vast majority of T cell epitopes discovered during the past two decades was identified by means of epitope prediction and MHC ligand identification. Here we describe the steps which are necessary to identify MHC epitopes from monoallelic and multiallelic cells and establish MHC class I peptide motifs to compose a reliable scoring matrix for epitope prediction. As an example, the ligands of monoallelic C1R cells and multiallelic peripheral blood mononuclear cell tissue will be identified and a scoring matrix for the prediction of HLA-C*01:02-presented T cell epitopes will be developed.