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Alternating Magnetic Field-Responsive Hybrid Gelatin Microgels for Controlled Drug Release
Published on: February 13, 2016
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Gelatin Nanoparticles
Rania M Hathout1, Abdelkader A Metwally2,3
1Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt. r_hathout@yahoo.com.
Methods in Molecular Biology (Clifton, N.J.)
|June 1, 2019
Summary
Gelatin nanoparticles offer versatile drug and gene delivery. The double-desolvation method provides a reliable technique for preparing homogenous nanoparticles, with protocols for cationization and FITC labeling also detailed.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Pharmaceutical Sciences
Background:
- Gelatin nanoparticles are increasingly utilized for drug and gene delivery across various administration routes.
- Challenges in preparing homogenous and well-dispersed gelatin nanoparticles stem from variations in gelatin source and molecular weight.
Purpose of the Study:
- To detail the double-desolvation method for optimal gelatin nanoparticle preparation.
- To present protocols for cationization of gelatin nanoparticles for genetic material or protein loading.
- To outline step-by-step procedures for FITC labeling of gelatin nanoparticles for imaging and biodistribution studies.
Main Methods:
- Detailed description of the double-desolvation method for nanoparticle synthesis.
- Protocols for cationization of prepared gelatin nanoparticles.
- Step-by-step FITC labeling technique for gelatin nanoparticles.
Main Results:
- The double-desolvation method yields optimum results in terms of particle size and homogeneity.
- Cationization protocols are established for enhanced loading of genetic materials and proteins.
- FITC labeling procedures are provided for imaging and biodistribution applications.
Conclusions:
- The double-desolvation method is a robust technique for producing high-quality gelatin nanoparticles.
- Established cationization and labeling protocols expand the utility of gelatin nanoparticles in biomedical applications.
- This chapter provides a comprehensive guide for researchers working with gelatin nanoparticles for drug and gene delivery.
