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Updated: Jan 24, 2026

Optical Trapping of Nanoparticles
13:39

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Optical Trapping in Plant Cells.

Tijs Ketelaar1, Norbert de Ruijter2, Stefan Niehren3

  • 1Laboratory of Cell Biology, Wageningen University, Wageningen, The Netherlands. tijs.ketelaar@wur.nl.

Methods in Molecular Biology (Clifton, N.J.)
|June 1, 2019
PubMed
Summary

Optical tweezers enable noninvasive manipulation and force measurements of subcellular components in living cells. This chapter details integrating optical tweezers with confocal microscopy for intracellular studies in plant cells.

Keywords:
Confocal microscopeCytoskeletonEndomembrane systemNoninvasive manipulationOptical trapOptical tweezersPlant cell

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Area of Science:

  • Biophysics
  • Cell Biology
  • Microscopy

Background:

  • Optical tweezers offer noninvasive manipulation of subcellular compartments.
  • Force measurements can be performed by analyzing displacements within living cells.

Purpose of the Study:

  • Provide practical guidance for setting up optical tweezers experiments.
  • Detail technical considerations for integrating optical tweezers with confocal microscopy.
  • Describe experimental methods for trapping intracellular structures in plant cells.

Main Methods:

  • Integration of optical tweezers into a confocal microscope setup.
  • Utilizing displacement measurements for semi-quantitative force analysis.
  • Developing experimental approaches for trapping intracellular components.

Main Results:

  • Successful trapping of intracellular structures in plant cells.
  • Demonstration of practical considerations for experimental setup.
  • Establishment of methods for in vivo force measurements.

Conclusions:

  • Optical tweezers are a valuable tool for studying physical interactions of subcellular components.
  • Confocal microscopy integration enhances the capabilities of optical tweezers for cellular studies.
  • The described methods facilitate advanced research in plant cell biophysics.