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Updated: Jan 24, 2026

Glycan Profiling of Plant Cell Wall Polymers using Microarrays
Published on: December 17, 2012
Plant Cell Lines in Cell Morphogenesis Research: From Phenotyping to -Omics
Petr Klíma1, Vojtěch Čermák2, Miroslav Srba2
1Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czech Republic.
Abstract:
Here we provide an overview of procedures for long-term cultivation, phenotyping, genotyping, and genetic transformation of cell cultures of tobacco cell lines BY-2 and VBI-0, and of A. thaliana, ecotype Landsberg erecta (LE) cell line. Notably, we present an improved protocol for BY-2 transformation and cloning and extend the available plant cell lines methodology toward high-throughput technologies like fluorescent-based cell sorting and transcriptomics.
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