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Updated: Jan 24, 2026
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Glycan Profiling of Plant Cell Wall Polymers using Microarrays
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Plant Cell Lines in Cell Morphogenesis Research: From Phenotyping to -Omics
Petr Klíma1, Vojtěch Čermák2, Miroslav Srba2
1Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czech Republic.
Methods in Molecular Biology (Clifton, N.J.)
|June 1, 2019
Summary
This study details methods for cultivating and analyzing tobacco (Nicotiana tabacum) BY-2 and VBI-0, and Arabidopsis thaliana Landsberg erecta (LE) cell lines. It introduces an improved tobacco BY-2 transformation protocol, enhancing plant cell line research.
Area of Science:
- Plant cell biology
- Molecular biology
- Biotechnology
Background:
- Establishing robust protocols for plant cell line cultivation and analysis is crucial for advancing plant science.
- Standardized methods are needed for long-term cell culture, phenotyping, genotyping, and genetic transformation.
- Existing methodologies may not fully support high-throughput applications.
Purpose of the Study:
- To provide a comprehensive overview of procedures for the long-term cultivation, phenotyping, genotyping, and genetic transformation of specific plant cell lines.
- To present an improved protocol for the genetic transformation and cloning of tobacco BY-2 cell lines.
- To extend existing plant cell line methodologies towards high-throughput technologies.
Main Methods:
- Long-term cultivation of tobacco BY-2 and VBI-0 cell lines, and Arabidopsis thaliana ecotype Landsberg erecta (LE) cell line.
- Phenotyping and genotyping techniques applied to these cell lines.
- Development and refinement of genetic transformation protocols, particularly for BY-2 cells.
- Adaptation of methods for high-throughput applications such as fluorescent-based cell sorting and transcriptomics.
Main Results:
- Established and optimized procedures for the long-term maintenance and characterization of tobacco and Arabidopsis cell lines.
- An improved protocol for tobacco BY-2 transformation and cloning was successfully developed and validated.
- The methodology was successfully extended to accommodate high-throughput analyses, including cell sorting and transcriptomics.
Conclusions:
- The presented procedures offer a robust framework for plant cell line research, supporting both fundamental studies and high-throughput applications.
- The improved BY-2 transformation protocol facilitates more efficient genetic manipulation of this widely used model system.
- These advancements contribute to the broader toolkit available for plant cell biology and biotechnology research.
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