Related Experiment Video
Updated: Jan 24, 2026

Fractionation of Lignocellulosic Biomass using the OrganoCat Process
Published on: June 5, 2021
Screening for Oily Yeasts Able to Convert Hydrolysates from Biomass to Biofuels While Maintaining Industrial Process
Patricia J Slininger1, Bruce S Dien2, Joshua C Quarterman2
1Bioenergy Research Unit, National Center for Agricultural Utilization Research, USDA-ARS, Peoria, IL, USA. Pat.Slininger@ars.usda.gov.
Abstract:
Research has recently intensified to discover new oleaginous yeast strains able to function quickly and efficiently in low-cost lignocellulosic hydrolysates to produce high-quality lipids for use in biodiesel and chemicals. Detailed techniques are given here for ranking candidate yeast strains based on conversion of hydrolysate sugars to lipids and then optimizing cultivation conditions for best performers in a 96-well aerobic microcultivation format. A full battery of assays applicable to high throughput of small-volume samples are described for efficiently evaluating cell biomass production, lipid accumulation, fatty acid composition, and sugar utilization. Original data is additionally presented on the validation of the microtechnique for GC analysis of lipid composition in yeast since this application involved modification of a previously published assay for microalgae.
More Related Videos
Related Concept Videos
Yeast Signaling
Microorganisms in Agriculture and Food industry
Antihypertensive Drugs: Angiotensin-Converting Enzyme Inhibitors
Design Example: Maintaining Level of an Embankment
Role of Vitamins in Maintaining Bone Health
Vitamin A
Vitamin A is involved in the process of bone remodeling. Retinoic acid, the active metabolite of Vitamin A, has nuclear receptors in osteoblasts and osteoclasts, which are involved in bone remodeling.
Vitamin B12
Vitamin B12 acts as a cofactor during the formation of osteoblast-related proteins, such as osteocalcin. Vitamin B12 plays a role...
Clinically Relevant Drug Product Specifications: Methods of Establishment

