Related Experiment Video
Updated: Jan 24, 2026

In vitro Reconstitution of the Active T. castaneum Telomerase
Published on: July 14, 2011
RESISTIN EFFECT ON TELOMERASE GENE EXPRESSION IN GASTRIC CANCER CELL LINE AGS
M Mohammadi1, M Hedayati1, N Zarghami2
1Shahid Beheshti University of Medical Sciences, Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Tehran, Islamic Republic of Iran.
Resistin promotes gastric cancer cell growth and increases human telomerase reverse transcriptase (hTERT) gene expression. These findings suggest Resistin signaling may be a therapeutic target for gastric cancer treatment.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Resistin, an adipokine, is elevated in gastric cancer patients.
- Its precise role in gastric carcinogenesis remains unclear.
- This study investigates Resistin's impact on gastric cancer progression.
Purpose of the Study:
- To evaluate Resistin's effect on gastric cancer cell proliferation.
- To assess Resistin's influence on telomerase gene expression in gastric cancer cells.
Main Methods:
- Utilized XTT assay and trypan blue staining to measure AGS cell proliferation.
- Quantified human telomerase reverse transcriptase (hTERT) mRNA expression via real-time RT-PCR.
Main Results:
- Exogenous Resistin dose-dependently increased gastric cancer cell proliferation and viability.
- Resistin treatment upregulated hTERT gene expression within 24 hours.
Conclusions:
- Resistin enhances gastric cancer cell proliferation and hTERT expression.
- Findings elucidate Resistin's role in gastric carcinogenesis.
- Targeting Resistin signaling could be a potential gastric cancer therapy.
More Related Videos
10:34Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
Published on: April 14, 2010
09:24Combined Conditional Knockdown and Adapted Sphere Formation Assay to Study a Stemness-Associated Gene of Patient-derived Gastric Cancer Stem Cells
Published on: May 9, 2020
Related Concept Videos
Telomeres and Telomerase
Telomeres and Telomerase
Cell Specific Gene Expression
Cell Specific Gene Expression
What is Gene Expression?
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
What is Gene Expression?