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DECREASED FERTILIZATION: HUMAN SPERM DNA FRAGMENTATION AND IN VITRO MATURATION OF OOCYTE IN STIMULATED ICSI CYCLES
T Haghpanah1, T Eslami-Arshaghi2, M R Afarinesh3
1Kerman University of Medical Sciences, Faculty of Medicine, Dept. of Anatomy, Afzalipour, Kerman, Iran.
Acta Endocrinologica (Bucharest, Romania : 2005)
|June 1, 2019
Summary
High sperm DNA fragmentation (SDF) negatively impacts fertilization rates and increases sperm nuclear undecondensation in in vitro matured oocytes following intracytoplasmic sperm injection (ICSI). This affects assisted reproductive technologies outcomes.
Area of Science:
- Reproductive Biology
- Andrology
- In Vitro Fertilization
Background:
- Sperm DNA fragmentation (SDF) is a critical factor affecting male fertility.
- Intracytoplasmic sperm injection (ICSI) is a widely used assisted reproductive technology.
- The impact of SDF on ICSI outcomes using germinal vesicle (GV) oocytes from stimulated cycles requires further elucidation.
Purpose of the Study:
- To investigate the correlation between SDF levels and fertilization rates.
- To assess the effect of SDF on sperm nuclear decondensation post-ICSI.
- To evaluate these parameters in cumulus-free GV oocytes matured in vitro (IVM) from stimulated cycles.
Main Methods:
- GV oocytes were retrieved and cultured for in vitro maturation (IVM).
- Oocytes were injected with processed semen, categorized by SDF levels (≤30% vs. >30%).
- Fertilization and sperm nuclear decondensation were assessed post-ICSI.
Main Results:
- A significantly lower fertilization rate was observed in the high SDF group (SDF > 30%) compared to the low SDF group (SDF ≤ 30%).
- The high SDF group showed a significantly higher percentage of unfertilized oocytes with undecondensed sperm heads (53%) versus the low SDF group (25%).
- Maturation rate to metaphase II was 69% (101 out of 146 GV oocytes).
Conclusions:
- Sperm DNA fragmentation significantly impairs fertilization rates in IVM oocytes.
- Elevated SDF levels are associated with increased instances of sperm nuclear undecondensation.
- These findings highlight the detrimental effect of SDF on ICSI success in specific clinical scenarios.
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