Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Infrared (IR) Spectroscopy: Overview01:09

Infrared (IR) Spectroscopy: Overview

4.7K
When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
4.7K
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

1.2K
Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
1.2K
Glassware Calibration01:11

Glassware Calibration

1.3K
Accurate calibration of glassware, such as volumetric flasks, pipettes, and burettes, is essential to ensure accurate measurements in the analytical laboratory. Calibration helps maintain consistency across measurements and prevents errors arising from inaccurate volumes.
Volumetric flasks: Volumetric flasks are designed to prepare aqueous solutions of precise volumes accurately with a calibration line on the neck. To calibrate a volumetric flask, it is important to fill it with distilled...
1.3K
Instrument Calibration01:12

Instrument Calibration

693
Instrument calibration is essential for ensuring that instruments produce accurate and consistent results. It is vital in manufacturing, healthcare, testing laboratories, and scientific research. Calibration processes are specific to each instrument and help enhance data accuracy. Each instrument has a unique calibration process tailored to its design and function to improve data accuracy.
Analytical Balance Calibration
An analytical balance measures mass and requires regular calibration to...
693
Plotting and Calibrating the Root Locus01:19

Plotting and Calibrating the Root Locus

453
Root loci often diverge as system poles shift from the real axis to the complex plane. Key points in this transition are the breakaway and break-in points, indicating where the root locus leaves and reenters the real axis. The branches of the root locus form an angle of 180/n degrees with the real axis, where n is the number of branches at a breakaway or break-in point.
The maximum gain occurs at the breakaway points between open-loop poles on the real axis, while the minimum gain is...
453
Calibration Curves: Correlation Coefficient01:10

Calibration Curves: Correlation Coefficient

4.6K
In a linear calibration curve, there is a value called the calibration coefficient, denoted by 'r,' which measures the strength and the direction of association between two variables. The correlation coefficient value ranges from −1 to +1. A value of +1 indicates a perfect positive linear correlation, −1 denotes a perfect negative correlation, and 0 implies no correlation between the two variables. A positive correlation value establishes that as one variable increases, the...
4.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same authorSame journal

Monte Carlo-based correction of geometric and instrumental biases in single-distance time-domain near-infrared spectroscopy on skeletal muscle.

Biomedical optics express·2026
Same author

A Novel Bioluminescence Tomography System Compatible with CBCT-guided Small Animal Irradiators for High-Precision Preclinical Radiation Research.

Radiation research·2026
Same author

Ocular diagnostics and occipital neurovascular coupling in ocular hypertension and open angle glaucoma.

Frontiers in neuroscience·2025
Same author

Approaches for modelling autocorrelation function and data processing in time-domain diffuse correlation spectroscopy.

Biomedical optics express·2025
Same author

Evaluating task-evoked neurovascular coupling using integrated OPM-MEG and fNIRS imaging.

NeuroImage·2025
Same author

Feasibility of diffuse Raman spectroscopy to detect in-vivo molecular changes in the tissue induced by subcutaneous implants.

Biomedical optics express·2025

Related Experiment Video

Updated: Jan 24, 2026

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
10:03

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Published on: June 27, 2014

18.4K

Self-calibrating time-resolved near infrared spectroscopy.

Stanislaw Wojtkiewicz1, Anna Gerega2, Marta Zanoletti3

  • 1School of Computer Science, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.

Biomedical Optics Express
|June 1, 2019
PubMed
Summary

This study introduces a self-calibrating method for time-resolved near-infrared spectroscopy, eliminating the need for instrument response function measurements. This approach accurately recovers tissue optical properties using multi-wavelength data.

More Related Videos

Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures
08:53

Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures

Published on: October 9, 2012

18.2K
Millifluidics for Chemical Synthesis and Time-resolved Mechanistic Studies
12:55

Millifluidics for Chemical Synthesis and Time-resolved Mechanistic Studies

Published on: November 27, 2013

11.7K

Related Experiment Videos

Last Updated: Jan 24, 2026

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
10:03

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Published on: June 27, 2014

18.4K
Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures
08:53

Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures

Published on: October 9, 2012

18.2K
Millifluidics for Chemical Synthesis and Time-resolved Mechanistic Studies
12:55

Millifluidics for Chemical Synthesis and Time-resolved Mechanistic Studies

Published on: November 27, 2013

11.7K

Area of Science:

  • Biomedical Optics
  • Spectroscopy
  • Medical Physics

Background:

  • Time-resolved near-infrared spectroscopy (TR-NIRS) is crucial for measuring absolute tissue optical properties.
  • Accurate characterization of the instrument response function (IRF) is essential for TR-NIRS data analysis.
  • Conventional methods require IRF measurements to separate instrument effects from tissue properties.

Purpose of the Study:

  • To develop a novel, self-calibrating analysis methodology for TR-NIRS data.
  • To eliminate the influence of the instrument response function from the analysis.
  • To enable direct spectral parameter recovery without prior instrument calibration.

Main Methods:

  • A new data analysis approach was developed to remove the IRF's influence.
  • The methodology utilizes multi-wavelength data from TR-NIRS instruments.
  • Phantom and in-vivo data were analyzed using the proposed self-calibrating method.

Main Results:

  • The self-calibrating approach demonstrated accurate recovery of optical properties and constituent concentrations.
  • Parameter recovery was within 10% compared to conventional curve fitting methods.
  • Introducing spectral constraints improved the reconstruction accuracy.

Conclusions:

  • The proposed methodology allows for direct parameter recovery from multi-wavelength TR-NIRS data without prior IRF measurement.
  • This self-calibrating approach simplifies TR-NIRS analysis and maintains high accuracy.
  • The method offers a more efficient and robust way to obtain absolute tissue optical properties.