Enzyme characterization and biological activities of a resuscitation promoting factor from an oil degrading bacterium...

Resuscitation-promoting factors (Rpf) are a class of muralytic enzymes, which participate in recovery of dormant cells and promoting bacteria growth in poor media. In the present study the expression vector of the rpf-1 gene from an oil-degrading bacterium Rhodococcus erythropolis KB1 was constructed and expressed in Escherichia coli. The expressed protein was purified by Ni²⁺-affinity chromatography, and showed muralytic activity when measured with 4-methylumbelliferyl-β-D-N,N',N″-triacetyl chitotrioside as substrate. Addition of purified Rpf-1 to R. erythropolis culture efficiently improved bacterial cell growth. The purified protein also increased resuscitation of viable but nonculturable cells of R. erythropolis to culturable state. The conserved amino acid residues including Asp⁴⁵, Glu⁵¹, Cys⁵⁰, Thr⁶⁰, Gln⁶⁹, Thr⁷⁴, Trp⁷⁵ and Cys¹¹⁴ of the Rpf-1 were replaced with different amino acids. The mutant proteins were also expressed and purified with Ni²⁺-affinity chromatography. The muralytic activities of the mutant proteins decreased to different extents when compared with that of the wild type Rpf-1. Gln⁶⁹ was found to play the most important role in the enzyme activity, substitution of Gln⁶⁹ with lysine (Q69K) resulted in the greatest decrease of muralytic activity. The other amino acid residues such as Asp⁴⁵, Glu⁵¹, Cys⁵⁰ and Cys¹¹⁴ were also found to be very important in maintaining muralytic activity and biological function of the Rpf-1. Our results indicated that Rpf-1 from R. erythropolis showed muralytic activities and weak protease activity, but the muralytic activity was responsible for its growth promotion and resuscitation activity.