Related Experiment Video

Updated: Jan 24, 2026

siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells
10:43

siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells

Published on: May 24, 2014

11.8K

TRIM14 expression is regulated by IRF-1 and IRF-2

Jingang Cui1, Xiao Xu1, Yutong Li1

  • 1Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, China.

FEBS Open Bio
|June 1, 2019
PubMed

Insights

Interferon (IFN)-I induces TRIM14 production via its promoter. Interferon regulatory factors (IRFs) 1 and 2 bind the TRIM14 promoter, with IRF-1 crucial for IFN-I-induced transcription and IRF-2 for basal transcription.

Area of Science:

  • Immunology
  • Molecular Biology

Background:

  • Tripartite motif-containing 14 (TRIM14) is a mitochondrial adaptor protein involved in innate immune signaling and antiviral defense.
  • TRIM14 expression is upregulated by Type I Interferon (IFN-I), but the regulatory mechanism remains unclear.

Purpose of the Study:

  • To elucidate the transcriptional regulation of TRIM14 by IFN-I.
  • To identify key promoter elements and transcription factors involved in TRIM14 gene expression.

Main Methods:

  • Promoter analysis to identify functional elements (GC box, ISRE).
  • Electrophoretic mobility shift assays (EMSAs) or similar to assess transcription factor binding.
  • Gene knockdown experiments (e.g., siRNA) to evaluate the role of IRF-1 and IRF-2.

Main Results:

  • A GC box and an Interferon-Stimulated Response Element (ISRE) are essential for basal TRIM14 transcription.
  • IFN-I activates the TRIM14 promoter through the ISRE.
  • Interferon regulatory factor-1 (IRF-1) and IRF-2 bind to the TRIM14 promoter, activating transcription.
  • IRF-1 knockdown significantly reduces IFN-α-induced TRIM14 transcription.
  • IRF-2 is critical for basal TRIM14 transcription but has a minimal role in IFN-α-induced transcription.

Conclusions:

  • IRF-1 is a key mediator of IFN-I-induced TRIM14 transcription.
  • IRF-2 plays a vital role in maintaining basal TRIM14 expression levels.
  • Understanding TRIM14 regulation by IRFs provides insights into innate immune responses.
Keywords:
IRF-1IRF-2ISRETRIM14promoter

More Related Videos

Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 112:00

Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 1

Published on: April 8, 2009

10.7K
Cercosporin-Photocatalyzed [4+1]- and [4+2]-Annulations of Azoalkenes Under Mild Conditions07:12

Cercosporin-Photocatalyzed [4+1]- and [4+2]-Annulations of Azoalkenes Under Mild Conditions

Published on: July 17, 2020

6.7K

Related Concept Videos

Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
25.8K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

3.9K
Predicting Products: SN1 vs. SN202:27

Predicting Products: SN1 vs. SN2

Nucleophilic substitution reactions of alkyl halides can proceed via an SN1 or an SN2 mechanism. While in SN2 reactions, the nucleophile attacks the substrate simultaneously as the leaving group departs, in SN1 reactions, the substrate first dissociates to give the carbocation intermediate. Various factors such as the structure of the substrate, the strength of the nucleophile, and the nature of the solvent promote one mechanism over the other.
With increased substitution on the alkyl halide,...
15.9K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.3K
Constitutive and Regulated Gene Expression01:27

Constitutive and Regulated Gene Expression

Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...
986
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.7K