Nitrogen deposition sources and patterns in the Greater Yellowstone Ecosystem determined from ion exchange resin collectors, lichens, and isotopes

Over the past century, atmospheric nitrogen deposition (N_dep) has increased across the western United States due to agricultural and urban development, resulting in degraded ecosystem quality. Regional patterns of N_dep are often estimated by coupling direct measurements from large-scale monitoring networks and atmospheric chemistry models, but such efforts can be problematic in the western US because of complex terrain and sparse sampling. This study aimed not only to understand N_dep patterns in mountainous ecosystems but also to investigate whether isotope values of lichens and throughfall deposition can be used to determine N_dep sources, and serve as an additional tool in ecosystem health assessments. We measured N_dep amounts and δ¹⁵N in montane conifer forests of the Greater Yellowstone Ecosystem using canopy throughfall and bulk monitors and lichens. In addition, we examined patterns of C:N ratios in lichens as a possible indicator of lichen physiological condition. The isotopic signature of δ¹⁵N of N_dep helps to discern emission sources, because δ¹⁵N of NO_x from combustion tends to be high (-5 to +25‰) while NH_x from agricultural sources tends to be comparatively low (-40 to -10‰). Summertime N_dep increased with elevation and ranged from 0.26 to 1.66 kg ha^-1. N_dep was higher than expected in remote areas. The δ¹⁵N values of lichens were typically -15.3 to -10‰ suggesting agriculture as a primary emission source of deposition. Lichen %N, δ¹⁵N and C:N ratios can provide important information about N_dep sources and patterns over small spatial scales in complex terrain.