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Graphene Oxide-Based Nanostructured DNA Sensor.

Aditya Balaji1, Songlin Yang2, Jeslyn Wang3

  • 1Department of Biomedical Engineering, University of Western Ontario, London, ON N6A 5B9, Canada. abalaji2@uwo.ca.

Biosensors
|June 2, 2019
PubMed
Summary
This summary is machine-generated.

We developed a graphene oxide-based sensor for rapid and accurate detection of single-strand DNA. This fluorescence quenching sensor uses fluorescent magnetic nanoparticles (FMNPs) and achieves a detection limit of 0.12 µM for target DNA.

Keywords:
DNA sensorFRET quenching mechanismfluorescent magnetic core-shell nanoparticlesgraphene oxide

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Rapid DNA sequence detection is crucial for early-stage diagnosis and various scientific fields.
  • Existing methods may lack the speed, accuracy, or sensitivity required for detecting small DNA amounts.
  • Graphene oxide and fluorescent magnetic nanoparticles offer unique properties for biosensing applications.

Purpose of the Study:

  • To develop a novel graphene oxide-based fluorescence quenching sensor for sensitive and rapid detection of single-strand DNA.
  • To utilize fluorescent magnetic nanoparticles (FMNPs) as FRET donors and for magnetic separation.
  • To investigate the sensor's performance, including its detection limit and accuracy.

Main Methods:

  • Fabrication of fluorescent magnetic nanoparticles (FMNPs) with iron oxide core and fluorescent silica shell.
  • Modification of FMNPs with target DNA (DNA-t) via glutaraldehyde crosslinking.
  • Preparation of graphene oxide (GO) nanosheets and modification with complementary DNA (DNA-c).
  • Utilizing Förster Resonance Energy Transfer (FRET) for fluorescence quenching and magnetic separation for enhanced accuracy.

Main Results:

  • FMNPs exhibited desirable photoluminescence and magnetic properties, with an average size of 74 ± 6 nm and silica shell thickness of 30 ± 4 nm.
  • The sensor demonstrated fluorescence quenching in the presence of DNA-c modified GO, indicating successful FRET.
  • The system achieved a low detection limit of 0.12 µM for target DNA (DNA-t).
  • Magnetic separation capability of FMNPs enhanced sensor accuracy by isolating the sensing system.

Conclusions:

  • The developed graphene oxide-based sensor effectively detects small amounts of single-strand DNA with high speed and accuracy.
  • FMNPs serve as efficient FRET donors and facilitate magnetic separation, improving overall sensor performance.
  • This sensing system shows significant potential for applications requiring sensitive DNA detection, such as early-stage diagnostics.