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Preparation of Binary and Ternary Deep Eutectic Systems
Published on: October 31, 2019
Optimizing the ultrasound-assisted deep eutectic solvent extraction of flavonoids in common buckwheat sprouts
Ahmad Rois Mansur1, Nho-Eul Song2, Hae Won Jang2
1Korea Food Research Institute, Wanju 55365, Republic of Korea; Department of Food Biotechnology, Korea University of Science and Technology, Daejeon 34113, Republic of Korea.
Abstract:
In this study, deep eutectic solvents (DESs) coupled with ultrasound-assisted extraction (UAE) were evaluated and optimized for the extraction of major flavonoids from common buckwheat sprouts. Initially, 18 different choline chloride (CC)-based DESs were evaluated as potential extraction solvents, and 80% CCTG (CC-based DES composed of triethylene glycol and 20 vol% water) extracted significantly higher (p < 0.05) amounts of flavonoids than other DESs studied. The extraction efficiency of 80% CCTG was even higher (p < 0.05) than that of methanol for the extraction of vitexin and quercetin-3-O-robinobioside. Procedure using 80% CCTG and UAE were optimized; an extraction temperature of 56 °C and extraction time of 40 min were considered optimal. The optimized extraction procedure was reliable and efficient for the extraction of major flavonoids from common buckwheat sprouts. In addition, flavonoids could be efficiently recovered from DES extracts with high recovery yields (>97%) by using a C18 solid-phase extraction.
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