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Related Concept Videos

Transcription01:10

Transcription

155.8K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
155.8K
Transcription Factors02:16

Transcription Factors

82.3K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
82.3K
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

25.2K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
25.2K
Eukaryotic Transcription Activators02:42

Eukaryotic Transcription Activators

12.6K
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
12.6K
Transcription Elongation Factors02:35

Transcription Elongation Factors

13.5K
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
13.5K
Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

18.2K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
18.2K

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Related Experiment Video

Updated: Jan 24, 2026

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy
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SplintQuant: a method for accurately quantifying circular RNA transcript abundance without reverse transcription

Vanessa Conn1,2, Simon J Conn1,2

  • 1Flinders Centre for Innovation in Cancer, Flinders University, College of Medicine and Public Health, Bedford Park, South Australia 5042, Australia.

RNA (New York, N.Y.)
|June 2, 2019
PubMed
Summary

Accurate RNA quantification is challenging. SplintQuant precisely measures circular RNAs (circRNAs) using DNA probes and qPCR, avoiding reverse transcription biases for reliable results.

Keywords:
circular RNAqPCRreverse transcriptionsplint ligation

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biochemistry

Background:

  • Reverse transcription of RNA can introduce errors, affecting transcript abundance quantification.
  • Circular RNAs (circRNAs) are prone to overestimation due to their structure, complicating accurate measurement.

Purpose of the Study:

  • To develop a novel method, SplintQuant, for accurate quantification of circular RNAs (circRNAs).
  • To overcome limitations of reverse transcription-based RNA quantification methods.

Main Methods:

  • SplintQuant utilizes custom DNA oligonucleotides ligated by PBCV-1 DNA ligase upon binding to target RNA.
  • These DNA oligonucleotides are tagged with universal primers for quantitative PCR (qPCR).
  • The method operates in the absence of reverse transcription, enhancing specificity.

Main Results:

  • SplintQuant accurately quantifies even lowly abundant circRNAs.
  • The method demonstrates high sensitivity, specificity, and reproducibility.
  • SplintQuant is applicable to various RNA transcripts, including canonical and noncanonical forms.

Conclusions:

  • SplintQuant offers a gold-standard approach for precise circRNA quantification.
  • The method provides a reliable alternative to traditional reverse transcription-based techniques.
  • SplintQuant enables accurate analysis of diverse RNA species, including splice variants and gene fusions.