Related Experiment Video
Updated: Jan 24, 2026
12:31
A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
Published on: February 28, 2015
15.6K
Development of a structure-switching aptamer-based nanosensor for salicylic acid detection
Changtian Chen1, Silu Feng2, Mian Zhou3
1Department of Plant Pathology and Microbiology, Iowa State University, Ames, IA, USA.
Biosensors & Bioelectronics
|June 2, 2019
Summary
A new sensor detects salicylic acid (SA) using aptamers and nanopore technology. This cost-effective method offers rapid, sensitive, and simple detection of SA in various samples, improving upon existing techniques.
Area of Science:
- Biotechnology
- Biosensing
- Plant Science
Background:
- Salicylic acid (SA) is a crucial plant hormone involved in immune responses against pathogens.
- SA and its derivatives are present in food, medicine, and cosmetics, with potential health benefits and risks.
- Current SA detection methods are expensive, time-consuming, and require specialized equipment.
Purpose of the Study:
- To develop a cost-effective, rapid, sensitive, and simple sensor for salicylic acid (SA) detection.
- To overcome challenges in aptamer selection for small molecules like SA.
- To enable on-site detection of SA in plant extracts.
Main Methods:
- Developed a structure-switching aptamer-based nanopore thin film sensor.
- Employed a structure-switching SELEX approach, immobilizing the library for aptamer selection.
- Validated the sensor's performance using buffer and plant extracts.
Main Results:
- Identified a high-affinity SA aptamer through a modified SELEX process.
- Achieved a detection limit as low as 0.1 μM SA, surpassing antibody-based methods.
- Demonstrated good selectivity and successful detection in Arabidopsis and rice extracts within 30 minutes.
Conclusions:
- The aptamer-based nanopore thin film sensor offers a promising solution for SA detection.
- This technology provides a low-cost, rapid, and sensitive method for on-site SA analysis.
- The developed sensor advances capabilities for monitoring plant health and related applications.
Related Concept Videos
03:05Acid Strength and Molecular Structure
32.9K
Binary Acids and Bases
In the absence of any leveling effect, the acid strength of binary compounds of hydrogen with nonmetals (A) increases as the H-A bond strength decreases down a group in the periodic table. For group 17, the order of increasing acidity is HF < HCl < HBr < HI. Likewise, for group 16, the order of increasing acid strength is H2O < H2S < H2Se < H2Te. Across a row in the periodic table, the acid strength of binary hydrogen compounds increases with increasing...
In the absence of any leveling effect, the acid strength of binary compounds of hydrogen with nonmetals (A) increases as the H-A bond strength decreases down a group in the periodic table. For group 17, the order of increasing acidity is HF < HCl < HBr < HI. Likewise, for group 16, the order of increasing acid strength is H2O < H2S < H2Se < H2Te. Across a row in the periodic table, the acid strength of binary hydrogen compounds increases with increasing...
32.9K
02:33Lewis Acids and Bases
48.2K
In 1923, G. N. Lewis proposed a generalized definition of acid-base behavior in which acids and bases are identified by their ability to accept or to donate a pair of electrons and form a coordinate covalent bond.
A coordinate covalent bond (or dative bond) occurs when one of the atoms in the bond provides both bonding electrons. For example, a coordinate covalent bond occurs when a water molecule combines with a hydrogen ion to form a hydronium ion. A coordinate covalent bond also results when...
A coordinate covalent bond (or dative bond) occurs when one of the atoms in the bond provides both bonding electrons. For example, a coordinate covalent bond occurs when a water molecule combines with a hydrogen ion to form a hydronium ion. A coordinate covalent bond also results when...
48.2K
02:54Ions as Acids and Bases
26.2K
Salts with Acidic Ions
Salts are ionic compounds composed of cations and anions, either of which may be capable of undergoing an acid or base ionization reaction with water. Aqueous salt solutions, therefore, may be acidic, basic, or neutral, depending on the relative acid-base strengths of the salt’s constituent ions. For example, dissolving the ammonium chloride in water results in its dissociation, as described by the equation:
Salts are ionic compounds composed of cations and anions, either of which may be capable of undergoing an acid or base ionization reaction with water. Aqueous salt solutions, therefore, may be acidic, basic, or neutral, depending on the relative acid-base strengths of the salt’s constituent ions. For example, dissolving the ammonium chloride in water results in its dissociation, as described by the equation:
26.2K
01:58Fruit Development, Structure, and Function
25.1K
Fruits form from a mature flower ovary. As seeds develop from the ovules contained within, the ovary wall undergoes a series of complex changes to form fruit. In some fruits, such as soybeans, the ovary wall dries; in other fruits, such as grapes, it remains fleshy. In some cases, organs other than the ovary contribute to fruit formation; such fruits are called accessory fruits.
25.1K
02:33Seed Structure and Early Development of the Sporophyte
30.9K
Seed structures are composed of a protective seed coat surrounding a plant embryo, and a food store for the developing embryo. The embryo contains the precursor tissues for leaves, stem, and roots. The endosperm and cotyledons—seed leaves—act as the food reserves for the growing embryo.
30.9K
02:34Molecular Structure and Acidity
20.5K
An acid can be deprotonated to form a conjugate base or an anion. If the produced anion is more stable, then the acid is stronger. On the contrary, if the anion is unstable, then the acid is weaker. Hence, to determine the acidity of the compound, the stability of its conjugate base is studied using various factors.
The size effect explains the change in atomic size on acidity. When comparing the acids formed from elements that belong to the same column in the periodic table, their atomic sizes...
The size effect explains the change in atomic size on acidity. When comparing the acids formed from elements that belong to the same column in the periodic table, their atomic sizes...
20.5K